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General description
Transcription factor A, mitochondrial (UniProt: Q00059; also known as mtTFA, Mitochondrial transcription factor 1, MtTF1, Transcription factor 6, TCF-6, Transcription factor 6-like 2, TFAM) is encoded by the TFAM (also known as TCF6, TCF6L2) gene (Gene ID: 7019) in human. TFAM is a DNA binding protein that plays multiple roles in regulating mitochondrial DNA function. As a sequence-specific transcription factor, it binds upstream of the light strand promoter and heavy strand promoter 1 to activate initiation of transcription. It is a component of the mitochondrial transcription initiation complex (composed of TFB2M, TFAM and POLRMT). In this complex, TFAM recruits POLRMT to the promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. TFAM contains two HMG boxes (aa 50-118 and 155-219). Phosphorylation by protein kinase A within the HMG box 1 is shown to impair its DNA binding and promotes its degradation by AAA+ Lon protease. Mutations in TFAM gene are known to cause hepatocerebral type of mitochondrial DNA depletion syndrome that is characterized by severe intrauterine growth restriction, neonatal-onset hypoglycemia and liver dysfunction. In this disease hepatic pathology includes cirrhosis, steatosis and cholestasis that leads to liver failure. (Ref.: Wang, YE., et al. (2013). PLoS ONE. 8(8); e74513).
Immunogen
Full-length recombinant human Transcription factor A, mitochondrial (TFAM).
Application
Quality Control Testing
Evaluated by Western Blotting in HeLa cell lysates.
Western Blotting Analysis: A 1:500 dilution of this antibody detected TFAM in HeLa cell lysates.
Tested Applications
ChIP-seq Analysis: A representative lot detected TFAM in ChIP-seq applications (Wang, Y.E., et. al. (2013). PLoS One. 8(8):e74513).
Immunoprecipitation Analysis: A representative lot immunoprecipitated TFAM in Immunoprecipitation applications (Wang, Y.E., et. al. (2013). PLoS One. 8(8):e74513).
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected TFAM in HeLa cells.
Western Blotting Analysis: A representative lot detected TFAM in Western Blotting applications (Wang, Y.E., et. al. (2013). PLoS One. 8(8):e74513).
Immunocytochemistry Analysis: A representative lot detected TFAM in Immunocytochemistry applications (Wang, Y.E., et. al. (2013). PLoS One. 8(8):e74513).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-TFAM, clone 20G2C12, Cat. No. MABE1994, is a mouse monoclonal antibody that detects Transcription factor A, mitochondrial (TFAM) and is tested for use in ChIP-seq, Immunocytochemistry, Immunoprecipitation, and Western Blotting.
Biochem/physiol Actions
Clone 20G2C12 is a mouse monoclonal antibody that detects human mitochondrial Transcription Factor A (TFAM).
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at +2°C to +8°C from date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 200 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 20G2C12, monoclonal |
| mol wt | calculated mol wt 21.59 kDa,observed mol wt ~25 kDa |
| species reactivity | human |
| packaging | antibody small pack of 100 μL |
| technique(s) | co-immunoprecipitation (co-IP): suitable,immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG1κ |
| UniProt accession no. | Q00059 |
| shipped in | dry ice |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... TFAM(7019) |
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¥ 1011.55
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