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General description
BIOCHEMISTRY: p I = 7.8
The previously assigned protein identifier P16189 has been merged into P04439. Full details can be found on the UniProt database.
Immunogen
Nalm-6 cell line
Application
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
This Anti-HLA-ABC Common Antibody, clone 22.63.4 is validated for use in FC, IC, IH for the detection of HLA-ABC.
This antibody is suitable for indirect immunofluorescence analysis by flow cytometry and is an excellent positive control. It is suitable for cryostat sections but not for routine formalin fixed paraffin sections. If the tissue is prepared at 4°C and fixed in PLP (Periodate-Lysine-Paraformaldehyde) before paraffin embedding, the antigen can be conserved (Hancock, 1982).
SUGGESTED USAGE DILUTION
1. Flow cytometry and indirect immunofluorescence 1:15 - 1:60
Dilute with isotonic buffer. Use 50 μl of diluted antibody per 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.
2. Indirect immunoperoxidase staining - the final dilution will depend on the assay conditions and detection system employed. However, a dilution of at least 1:15 will be applicable to most commonly used systems.
Biochem/physiol Actions
This antibody reacts with a common framework determinant of the 40-44kD protein of HLA-ABC Class I antigen. The antibody precipitates antigens of 40 - 44kD and 12kD (b2-microglobulin).
Cell reactivity:
The antibody reacts with all cells having HLA-ABC surface antigen. Erythrocytes and K562 and DAUDI are negative.
Physical form
Format: Purified
Purified from mouse ascitic fluid. The antibody is supplied in phosphate buffered saline, pH 7.4 containing 0.1% sodium azide, diluted 50% v/v with glycerol. The characteristics of each lot are tested by electrophoresis and flow cytometry.
Preparation Note
Store at 2 to 8°C, for up to 6 months. For prolonged periods, store at -15 to -20°C in undiluted aliquots. At this temperature the glycerol solution will not freeze.
WARNING: The monoclonal reagent solution contains 0.05% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MAB1280
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 22.63.4, monoclonal |
| species reactivity | human |
| manufacturer/tradename | Chemicon® |
| technique(s) | flow cytometry: suitable,immunocytochemistry: suitable,immunohistochemistry: suitable |
| isotype | IgG2b |
| NCBI accession no. | NM_002116.5 |
| UniProt accession no. | P04439 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... HLA-A(3105) |
| 长度(mm) | |
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