首页> 生物试剂> 其他生物试剂> 其他> Anti-Cyclin T1 Antibody，100UL，06-1289，Sigma

收藏产品

Anti-Cyclin T1 Antibody，100UL，06-1289，Sigma

销售价： ¥ 3306.88 / 件

在线询价

优惠：请询价

订货号： 0BV3689

品牌型号： Sigma 06-1289

货期：询货期

最小订货量： 1件

+ -

加入购物车

联系我们

400-920-3909

微信扫一扫，手机采购

精选商品

胰蛋白胨，500g，LP0042B，Oxoid

销售价：询价

加入购物车

商品介绍
规格参数
包装参数

产品介绍 Product Description

General description

Cyclin T1 is expected to be ~85 kDa This antibody detects a ~85 kDa band in most cell lines. It also detects the degraded forms of Cyclin T1 (~70 kDa, ~60 kDa, et al), which is beneficial for IP applications. According to independent laboratory, this antibody can immunoprecipitate multiple forms of Cyclin T1.
Cyclin T1, which is also known as CCNT, is a member of the cyclin family and functions as a CDK kinase regulator. Cyclin T1 is one of regulatory cyclins (T1, T2a, T2b, and K) that is closely associated with CDK9 kinase forming a heterodimer called P-TEFb. This kinase complex is involved in phosporylating the carboxyl-terminal domain (CTD) of the large RNA polymerase II subunit which stimulate the elongation upon initiation. The cyclin T1/CDK9 complex has also been referred to as tat-associated kinase. Tat is an HIV protein that targets the cyclin T1/CDK9 complex to activate the transcription of the virus.

Immunogen

Epitope: Unknown
His-tagged recombinant protein corresponding to human Cyclin T1.

Application

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Use Anti-Cyclin T1 Antibody (Sheep Polyclonal Antibody) validated in WB to detect Cyclin T1 also known as CDK9-associated C-type protein, cyclin C-related protein.
Western Blot Analysis: A 1:1000 dilution from a previous lot detected Cyclin T1 in 10 µg of HEK293, HeLa, HepG2, Jurkat, and PC3 cell lysates.

Biochem/physiol Actions

Rat (85% sequence homology).
This antibody recognizes Cyclin T1.

Physical form

Sheep polyclonal serum with 0.05% sodium azide.
Unpurified

Preparation Note

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
HeLa cell lysate
Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: A 1:1000 dilution of this antibody detected Cyclin T1 in 10 µg of HeLa cell lysate.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

技术参数 Specifications

biological source	sheep
Quality Segment	100
conjugate	unconjugated
antibody form	serum
antibody product type	primary antibodies
clone	polyclonal
species reactivity	human, mouse
technique(s)	western blot: suitable
isotype	IgG
NCBI accession no.	NP_001231.2
UniProt accession no.	O60563
shipped in	wet ice
target post-translational modification	unmodified
Gene Information	human ... CCNT1(904) mouse ... Ccnt1(12455)

长度(mm)
宽度(mm)
高度(mm)
重量(kg)