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General description
Small ubiquitin-related modifier 1 (UniProt: P63165; also known as SUMO-1, GAP-modifying protein 1, GMP1, SMT3 homolog 3, Sentrin, Ubiquitin-homology domain protein PIC1, Ubiquitin-like protein SMT3C, Smt3C, Ubiquitin-like protein UBL1) is encoded by the SUMO1 (also known as SMT3C, SMT3H3, UBL1, OK/SW-cl.43) gene (Gene ID: 7341) in human. SUMO-1 is a ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in many cellular processes, such as nuclear transport, DNA replication and repair, mitosis and signal transduction. SUMO-1 can also covalently attach to the voltage-gated potassium channel KCNB1; this modulates the gating characteristics of KCNB1. Polymeric SUMO-1 chains are shown to be susceptible to polyubiquitination, which functions as a signal for proteasomal degradation of modified proteins. Two isoforms of SUMO-1 have been reported that are produced by alternative splicing. Mutations in SUMO1 gene are known to cause non-syndromic orofacial cleft 10, which is a birth defect consisting of cleft lips with or without cleft palate.
~17 kDa observed; 11.56 kDa calculated. SUMO-1/RanGAP1 band is observed AT ~ 75 kDa Uncharacterized bands may be observed in some lysate(s).
Immunogen
His-tagged full length human recombinant Small ubiquitin-related modifier 1 (SUMO-1).
Application
Anti-SUMO-1, clone 21C7, Cat. No. MABS2071, is a mouse monoclonal antibody that detects Small ubiquitin-related modifier 1 (SUMO-1) and has been tested for use in Immunocytochemistry, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, and Western Blotting.
Immunohistochemistry Analysis: A representative lot detected SUMO-1 in Immunohistochemistry applications (Rogers, R.S., et. al. (2004). Chromosoma. 113(5):233-43; Costa, M.W., et. al. (2011). PLoA One. 6(9):e24812).
Immunocytochemistry Analysis: A representative lot detected SUMO-1 in Immunocytochemistry applications (Matunis, M.J., et. al. (1996). J Cell Biol. 135(6 Pt 1):1457-70; Zhang, X.D., et. al. (2008). Mol Cell. 29(6):729-41).
Immunoprecipitation Analysis: A representative lot immunopprecipitated SUMO-1 in Immunoprecipitation applications (Costa, M.W., et. al. (2011). PLoA One. 6(9):e24812; Becker, J., et. al. (2013). Nat Struct Mol Biol. 20(4):525-31; Girach, F., et. al. (2013). Cell Rep. 5(5):1294-301; Chanda, A., et. al. (2017). PLoS One. 12(5):e0177639).
Western Blotting Analysis: A representative lot detected SUMO-1 in Western Blotting applications (Zhang, X.D., et. al. (2008). Mol Cell. 29(6):729-41; Matunis, M.J., et. al. (1996). J Cell Biol. 135(6 Pt 1):1457-70; Rogers, R.S., et. al. (2004). Chromosoma. 113(5):233-43; Costa, M.W., et. al. (2011). PLoA One. 6(9):e24812; Becker, J., et. al. (2013). Nat Struct Mol Biol. 20(4):525-31).
Immunofluorescence Analysis: A representative lot detected SUMO-1 in Immunofluorescence applications (Girach, F., et. al. (2013). Cell Rep. 5(5):1294-301; Rogers, R.S., et. al. (2004). Chromosoma. 113(5):233-43; Matunis, M.J., et. al. (1996). J Cell Biol. 135(6 Pt 1):1457-70).
Research Category
Signaling
Biochem/physiol Actions
Clone 21C7 detects SUMO-1 in multiple species. It targets an epitope with in 11 amino acids from the internal region.
Physical form
Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in HCT116 cell lysate.
Western Blotting Analysis: 4 µg/mL of this antibody detected SUMO-1 in HCT116 cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 21C7, monoclonal |
| species reactivity | chicken, human, mouse, zebrafish, rat, Xenopus, fish |
| packaging | antibody small pack of 25 μg |
| technique(s) | immunocytochemistry: suitable,immunofluorescence: suitable,immunohistochemistry: suitable,immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG2b |
| NCBI accession no. | NP_001005781 |
| UniProt accession no. | P63165 |
| target post-translational modification | unmodified |
| Gene Information | human ... SUMO1(7341) |
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