Rosetta-gami B Competent Cell Set - Novagen,1SET,71177-3CN,Sigma

销售价: ¥ 3449.73 / 件
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订货号 0BV3648
品牌型号 Sigma 71177-3CN
货期 询货期
最小订货量 1件
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产品介绍 Product Description

General description

Genotypes:

Rosetta-gami B

FompT hsdSB (rB mB) gal dcm lacY1 ahpC gor522::Tn10 trxB pRARE (CamR, KanR, TetR)

Rosetta-gami B(DE3)

FompT hsdSB (rB mB) gal dcm lacY1 ahpC (DE3) gor522::Tn10 trxB pRARE (CamR, KanR, TetR)

Rosetta-gami B(DE3)pLysS

FompT hsdSB (rB mB) gal dcm lacY1 ahpC (DE3) gor522::Tn10 trxB pLysSRARE (CamR, KanR, TetR)

This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Rosetta-gami B strains combine the key features of BL21 (and its Tuner derivative), Origami, and Rosetta to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds in the bacterial cytoplasm. These strains are compatible with ampicillin- or spectinomycin-resistant vectors.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.


Other Notes

•2 × 0.2 mlRosetta-gami B Competent Cells

•2 × 0.2 mlRosetta-gami B(DE3) Competent Cells

•2 × 0.2 mlRosetta-gami B(DE3)pLysS Competent Cells

•4 × 2 mlSOC Medium

•2 ngTest Plasmid


Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany


Disclaimer

This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the “EMD Product”) requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
Toxicity: Multiple Toxicity Values, refer to MSDS (O)


技术参数 Specifications
biological sourceEscherichia coli
Quality Segment100
manufacturer/tradenameNovagen®
storage conditionOK to freeze,avoid repeated freeze/thaw cycles
growth modeadherent or suspension
morphologyrod shaped
technique(s)microbiological culture: suitable
shipped indry ice
storage temp.−70°C
长度(mm)
宽度(mm)
高度(mm)
重量(kg)
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