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General description
Protein prune homolog, otherwise known as PRUNE or Drosophila-related expressed sequence 17, is a phosphodiesterase enzyme belonging to the PPase class C family. PRUNE targets cAMP and cGMP second messengers and plays a role in cell proliferation and motility. It is overexpressed in various sarcomas, including leiomyosarcomas, malignant fibrous histiocytomas, and malignant liposarcomas.
~57 kDa observed
Immunogen
KLH-conjugated linear peptide corresponding to human PRUNE.
Application
Detect PRUNE using this Anti-PRUNE Antibody validated for use in Western Blotting.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HeLa S3 cell lysate
Evaluated by Western Blot in HeLa S3 cell lysate.
Western Blot Analysis: A 1:5,000 dilution of this antibody detected PRUNE in 10 µg of HeLa S3 cell lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human |
| technique(s) | western blot: suitable |
| NCBI accession no. | NP_067045 |
| UniProt accession no. | Q86TP1 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... PRUNE(58497) |
| 长度(mm) | |
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