Ready-to-Assay CCR9 Chemokine Receptor Frozen Cells，1KIT，HTS036RTA，Sigma

General description

Millipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

The CCR9 receptor is a GPCR expressed selectively in the thymus, lymph node, spleen, and the small bowel (SB). CCR9 binds to Thymus-expressed chemokine ligand (TECK / CCL25) (Zaballos et al. 1999, Yu et al., 2000, Carramolino et al., 2001), and is involved in directing the movement of mature T cells in the thymus. CCR9 also plays an important role in the pathogenesis of SB Crohn′s disease (Papadakis et al, 2001). The CCR9 selective expression in the small bowel but not the colon indicates the the role of CCR9 in the specialized immune response in the SB. It was also shown that CCR9-mediated antiapoptosis could help in the study of cell survival mechanism and T cell malfunctioning. Millipore′s cloned human CCR9-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant CCR9 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists, and modulators at CCR9.

Application

Human CCR9 GPCR frozen cells for Calcium Flux FLIPR Assays.

Biochem/physiol Actions

• GPCR Cell Lines
• Host cells: Chem-1

• GPCR Class: A
• Protein Target: CCR9
• Target Sub-Family: Chemokine

Other Notes

Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
Fifty (50) mL of Media Component.

Disclaimer

This product contains genetically modified organisms (GMO).
Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively.