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Anti-BAFF/CD257 Antibody, clone 5A8，100UL，MABF3094-100UL，Sigma

销售价： ¥ 3346.40 / 件

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订货号： 0BV3515

品牌型号： Sigma MABF3094-100UL

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最小订货量： 1件

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产品介绍 Product Description

General description

Tumor necrosis factor ligand superfamily member 13B (UniProt: Q9WU72; also known as B-cell-activating factor, BAFF, CD257) is encoded by the Tnfsf13b (also known as Baff) gene (Gene ID: 24099) in murine species. BAFF is a single-pass type II, homotrimeric, membrane glycoprotein with a cytoplasmic domain (aa 1-47), a transmembrane domain (aa 48-68), and an extracellular domain (aa 69-309). A soluble form of BAFF has also been described that is produced by the cleavage of membrane form between amino acids 126-127. It is expressed by cells of myeloid origin and by stromal cells. Two isoforms of BAFF have been described that are produced by alternative splicing. Isoform 2 is reported to inhibit the secretion and bioactivity of isoform 1. BAFF is reported to bind to TNFRSF13B/TACI and TNFRSF17/BCMA. It serves as a potent B cell survival factor and is a critical immune checkpoint during peripheral B cell maturation. Its levels are often elevated in autoimmune diseases, such as rheumatoid arthritis and systemic lupus erythematosus (SLE). Elevated levels of BAFF are also observed in subjects with non-Hodgkin s lymphoma (NHL) and it is reported to promote survival of both NHL-derived B lymphoma cells and B cell chronic lymphocytic leukemia (B-CLL) cells. (Ref.: Vigolo, M., et al. (2018). Nat. Commun. 9(1); 1199; Kowalczyk-Quintas, C., et al (2016). J. Biol. Chem. 291(38); 19826-19834; Batten, M., et al. (2004). J. Immunol. 172(2); 812-822; Gavin, AL., et al. (2003). J. Biol. Chem. 278(40); 38220-38228).

Immunogen

Rat RBL cells expressing full-length mouse BAFF.

Application

Quality Control Testing

Evaluated by Western Blotting with recombinant murine BAFF protein .

Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected recombinant murine BAFF/CD257 protein .

Tested Applications

Activity Assay: A representative lot activated mouse BAFF in a reporter cell assay, in primary B cells. (Kowalczyk-Quintas, C., et al. (2016). J Biol.Chem.291(38):19826-34; Vigolo, M., et al. (2018). Nat Commun.9(1):1199).

ELISA Analysis: A representative lot detected BAFF/CD257 in ELISA applications (Batten, M., et al. (2004). J Immunol. 172(2):812-22; Vigolo, M., et al. (2018). Nat Commun. 9(1):1199; Kowalczyk-Quintas, C., et al. (2016). J Biol Chem. 291(38):19826-34).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Biochem/physiol Actions

Clone 5A8 is a rat monoclonal antibody that detects B-cell activating factor (BAFF/CD257).

Physical form

Purified rat monoclonal antibody IgG1 in PBS without azide.

Preparation Note

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

技术参数 Specifications

biological source	rat
Quality Segment	200
conjugate	unconjugated
antibody form	purified antibody
antibody product type	primary antibodies
clone	5A8, monoclonal
species reactivity	mouse
packaging	antibody small pack of 100
technique(s)	activity assay: suitable,direct ELISA: suitable,western blot: suitable
isotype	IgG1κ
epitope sequence	Unknown
Protein ID accession no.	NP_001334238
UniProt accession no.	Q9WU72
target post-translational modification	unmodified
Gene Information	mouse ... Klrk1(24099)

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