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General description
Mediator of RNA polymerase II transcription subunit 25 (UniProt: Q71SY5; also known as Activator interaction domain-containing protein 1, Activator-recruited cofactor 92 kDa component, ARC92, Mediator complex subunit 25, p78) is encoded by the MED25 (also known as ACID1, ARC92, PTOV2, TCBAP0758) gene (Gene ID: 81857) in human. It is a member of the Mediator complex subunit 25 family that is ubiquitously expressed. Higher expression levels are observed in brain, heart, kidney, peripheral leukocytes, placenta, skeletal muscle and spleen. It acts as a coactivator that is involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator complex subunit 25 functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. It is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Six isoforms of Mediator complex subunit 25 have been described that are generated by alternative splicing. Defects in MED25 gene are known to cause Charcot-Marie-Tooth disease 2B2, a disorder of the peripheral nervous system characterized by progressive weakness and atrophy.
~70 kda observed; 78.17 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
KLH-conjugated linear peptide corresponding to 16 amino acids from the internal region of human Mediator of RNA polymerase II transcription subunit 25 (MED25).
Application
Anti-Mediator complex subunit 25, Cat. No. ABE1420, is a highly specific rabbit polyclonal antibody that targets MED25 and has been tested in Immunohistochemistry (Paraffin), Peptide Inhibition Assay, and Western Blotting.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Mediator complex subunit 25 in human cerebellum and mouse cerebral cortex tissues.
Peptide Inhibition Analysis: 2 µg/mL from a representative lot blocked Mediator complex subunit 25 in mouse brain tissue lysate.
Research Category
Epigenetics & Nuclear Function
Biochem/physiol Actions
This rabbit polyclonal antibody detects Mediator of RNA polymerase II transcription subunit 25 (MED25) in human and murine species. it targets an epitope within 16 amino acids from the internal region. Detects all isoforms of Mediator complex subunit 25.
Physical form
Affinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine, 0.15 M NaCl, pH 7.4 with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected Mediator complex subunit 25 in 10 µg of HeLa cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | mouse, human |
| species reactivity (predicted by homology) | rat (based on 100% sequence homology), yeast (based on 100% sequence homology), bovine (based on 100% sequence homology) |
| packaging | antibody small pack of 25 μg |
| technique(s) | immunohistochemistry: suitable (paraffin),inhibition assay: suitable (peptide),western blot: suitable |
| NCBI accession no. | NP_112235.2 |
| UniProt accession no. | Q71SY5 |
| shipped in | ambient |
| target post-translational modification | unmodified |
| Gene Information | human ... MED25(81857) |
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