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General description
Dendrin (UniProt: O94850) is encoded by the DDN (also known as KIAA0749) gene (Gene ID: 23109) in human. Dendrin is a proline-rich cytoplasmic protein that was originally recognized in telencephalic dendrites of sleep-deprived rats. Dendrin is specifically expressed in the brain and in kidneys. In the brain, it interacts with alpha-actinin and possibly modulates the structure of the actin cytoskeleton. In kidney it plays a role in apoptosis of kidney glomerular podocytes. Dendrin contains two putative nuclear localization signals (NLSs) and three PPXY motifs that are conserved among human, rat, and mouse. Dendrin is reported to interact with alpha-actinin and Maguk with inverted orientation (MAGI) or synaptic scaffolding molecule (S-SCAM). Interaction with S-SCAM is shown to be responsible for the retention of dendrin in the cytoplasm of mammalian cells and prevent its nuclear import. In experimental glomerulonephritis, dendrin is shown to relocate from the slit diaphragms (SD) to the nucleus of injured podocytes. High-doses of TGF- 1 can directly promote the import of dendrin into the nucleus and in the nucleus Dendrin enhances staurosporine- and TGF- 1-mediated apoptosis. Acute nicotine treatment is reported to induce a rapid, but short-lived, expression of the dendritically targeted, corticolimbic mRNA dendrin in specific regions of adolescent brain. (Ref.: Schochet, TL et al (2008). Eur.J. Neurosci. 28(10):1967-79; Kremerskothen, J et al. (2006). J. Neurochem. 96(6):1659-66; Asanuma, K et al. (2007). Proc. Natl. Acad. Sci. USA. 104(24): 10134-39).
~70 kDa observed, 76 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
KLH-conjugated linear peptide corresponding to 17 amino acids from the N-terminal region of human Dendrin.
Application
Anti-Dendrin Antibody, Cat. No. AB15299-I, is a highly specific rabbit polyclonal antibody that targets Dendrin and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Research Category
Neuroscience
Western Blotting Analysis: 2 µg/mL from a representative lot detected Dendrin in 10 µg of human hippocampus tissue lysate.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Dendrin in rat cerebral cortex, mouse cerebellum, and rat kidney tissue.
Biochem/physiol Actions
This polyclonal antibody recognizes Dendrin in human, mouse, and rat. It targets an epitope of 17 amino acids from the N-terminal region.
Physical form
Affinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in mouse hippocampus tissue lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected Dendrin in 10 µg of mouse hippocampus tissue lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Replaces: AB15299
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human, mouse, rat |
| technique(s) | immunohistochemistry: suitable (paraffin),western blot: suitable |
| NCBI accession no. | NP_055901 |
| UniProt accession no. | O94850 |
| shipped in | ambient |
| target post-translational modification | unmodified |
| Gene Information | human ... DDN(23109) |
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