- 商品介绍
- 规格参数
- 包装参数
General description
注意：1 mU = 1毫单位。
重组产气荚膜梭菌 α2-3，6-神经氨酸酶在大肠埃希菌中表达。对来自复合碳水化合物和糖蛋白类的非还原性末端α2，3和α2，6无支链唾液酸残基的水解进行催化。该酶对α2，8或支链唾液酸不显示活性。
Application
推荐的反应缓冲液：50mM磷酸钠缓冲液，pH 6.0
Physical form
在 25 mM NaCl，20 mM Tris-HCl，pH 7.5中。
Other Notes
Prime, S., et al. 1996.J. Chromatogr. A. 720, 263.
Dwek, R.A., et al. 1993.Annu.Rev. Biochem.62, 65.
Ohta, Y., et al. 1989.J. Biochem (Tokyo) 106, 1086.
一个单位定义为在37°C，pH值5.0下每分钟从2ʹ-（4-甲基伞形酮）-α-D-N-乙酰神经氨酸（目录号474495）催化释放1µmol甲基伞形酮的酶量。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
毒性：有害（C）
| recombinant | expressed in E. coli |
| Quality Level | 100 |
| description | RTECS - QQ3450000 |
| product line | EMPROVE® EXPERT |
| form | liquid |
| specific activity | ≥10 units/mL,≥250 units/mg protein |
| manufacturer/tradename | Calbiochem® |
| storage condition | do not freeze |
| foreign activity | N-acetylglucosaminidase, α-fucosidase, α- and β-galactosidase, α- and β-mannosidase, proteases, none detected |
| shipped in | wet ice |
| storage temp. | 2-8°C |
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