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- 规格参数
- 包装参数
General description
57 kDa
Immunogen
Vimentin purified from bovine lens
Application
Western blot
Immunohistochemistry: 1:100; Frozen and paraffin-embedded tissue sections with a minimum of a one hour incubation (longer for paraffin); protease pretreatment is recommended for paraffin-embedded sections.
Immunofluorescence
ELISA
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Research Sub Category
Cytoskeleton
This Anti-Vimentin Antibody, clone VIM 3B4 is validated for use in ELISA, IF, IH(P), WB for the detection of Vimentin.
Biochem/physiol Actions
Highly specific for the intermediate filament protein vimentin which is present in all cells of mesenchymal origin. This antibody is positive with cells of mesenchymal derivation, including endothelial cells and certain smooth muscle cells of the vascular tract, fibroblasts, connective tissue, all types of blood cells, including thymocytes, interstitial and testicular Sertoli cells, ovarian follicle cells. It can also be used to detect coexpression of vimentin in combination with other intermediate filament proteins. VIM 3B4 has turned out to be the most avid monoclonal antibody to vimentin. Tumors specifically detected: sarcoma (including myosarcoma), lymphoma, melanoma. The binding region of monoclonal antibody VIM3B4 has been characterized by Bohn et al. (1992). According to these authors, the epitope has been localized on the alpha-helical part of vimentin (rod domain coil 2). Due to an aa substitution at position of aa 353 in murine vimentin (that could explain for the weak cross-reaction of the antibody with murine vimentin) they were able to narrow down the binding region around position 353. These findings were confirmed by truncation mutagenesis experiments using human vimentin (Rogers et al., 1995). Polypeptide reacting: MW 57 kDa intermediate filament protein (vimentin) of mesenchymal cells.
Physical form
Format: Purified
Protein A Purified immunoglobulin presented as lyophilized material. Reconstitute with 1ml distilled water. Final solution is PBS, pH 7.4, containing 0.5% BSA and 0.09% sodium azide.
Protein A purified
Preparation Note
Maintain at 2–8°C for up to 12 months from date of receipt.
Analysis Note
Control
Positive in RD cells, glioma cells, fibroblasts (SV-80), and MDCK cell line
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | VIM 3B4, monoclonal |
| species reactivity | chicken, amphibian, bovine, monkey, canine, human |
| manufacturer/tradename | Chemicon® |
| technique(s) | ELISA: suitable,immunofluorescence: suitable,immunohistochemistry: suitable (paraffin),western blot: suitable |
| isotype | IgG2a |
| NCBI accession no. | NM_003380.2 |
| UniProt accession no. | P08670 |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| Gene Information | human ... VIM(7431) |
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