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General description
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
16 kDa Calculated
Immunogen
Epitope: methylated lysine 9
KLH-conjugated, synthetic 2X-branched peptide corresponding to histone H3 monomethylated at lysine 9.
Application
Detect monomethyl-Histone H3 (Lys9) using this Milli-Mark Anti-monomethyl-Histone H3 (Lys9)-FITC Antibody validated for use in FC.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histone Modifying Proteins
Histones
Biochem/physiol Actions
Broad species cross-reactivity is expected based on sequence homology.
Physical form
Protein A purified
Purified rabbit polyclonal IgG conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Preparation Note
Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
Analysis Note
Control
HeLa cells
Evaluated by flow cytometry using HeLa cells.
Legal Information
MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | FITC conjugate |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | human |
| manufacturer/tradename | Milli-Mark® |
| technique(s) | flow cytometry: suitable |
| isotype | IgG |
| NCBI accession no. | NP_003484 |
| UniProt accession no. | Q16695 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... H3C1(8350) |
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