Anti-Prdm9 Antibody, clone 1H9Z1, Rabbit Monoclonal，100UL，SAB5702253-100UL，Sigma 供应商、价格、厂家、报价、规格参数

产品介绍 Product Description

General description

Histone methyltransferase that sequentially mono-, di-, and tri-methylates both ′Lys-4′ (H3K4 and ′Lys-36′ (H3K36 of histone H3 to produce respectively trimethylated ′Lys-4′ (H3K4me3 and trimethylated ′Lys-36′ (H3K36me3 histone H3 and plays a key role in meiotic prophase by determining hotspot localization thereby promoting meiotic recombination. Also can methylate all four core histones with H3 being the best substrate and the most highly modified. Is also able, on one hand, to mono and di-methylate H4K20 and on other hand to trimethylate H3K9 with the di-methylated H3K9 as the best substrate. During meiotic prophase, binds specific DNA sequences through its zinc finger domains thereby determining hotspot localization where it promotes local H3K4me3 and H3K36me3 enrichment on the same nucleosomes through its histone methyltransferase activity. Thereby promotes double-stranded breaks (DSB formation, at this subset of PRDM9-binding sites, that initiates meiotic recombination for the proper meiotic progression.

Immunogen

PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Recombinant fusion protein containing a sequence corresponding to amino acids 87-370 of mouse Prdm9 (NP_659058.3).

Application

WB, IHC

技术参数 Specifications

biological source	rabbit
Quality Segment	100
conjugate	unconjugated
material	colorless
clone	1H9Z1, monoclonal
form	liquid
mol wt	110 kDa
species reactivity	human, rat
concentration	1 mg/mL
technique(s)	immunohistochemistry: 1:50 - 1:200,western blot: 1:500 - 1:1000
color	colorless
isotype	IgG
immunogen sequence	DSEDSDEEWTPKQQVSPPWVPFRVKHSKQQKESSRMPFSGESNVKEGSGIENLLNTSGSEHVQKPVSSLEEGNTSGQHSGKKLKLRKKNVEVKMYRLRERKGLAYEEVSEPQDDDYLYCEKCQNFFIDSCPNHGPPLFVKDSMVDRGHPNHSVLSLPPGLRISPSGIPEAGLGVWNEASDLPVGLHFGPYEGQITEDEEAANSGYSWLITKGRNCYEYVDGQDESQANWMRYVNCARDDEEQNLVAFQYHRKIFYRTCRVIRPGCELLVWYGDEYGQELGIKWG
UniProt accession no.	Q96EQ9
shipped in	wet ice
storage temp.	−20°C
target post-translational modification	unmodified
Gene Information	mouse ... Prdm9(213389)

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