Rat Dermal Fibroblasts: RDF, neonatal，1EACH，R106-05N，Sigma

General description

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RDF from Cell Applications, Inc. have been used in allogenic prosthetic grafts, demonstrating improved epithelization, collagen organization and faster regeneration (Nomoto, 2012), in a cell sheet engineering study, where a sheet of dermal fibroblasts was transplanted into the damaged liver in allogeneic rat model to promote migration of hepatic progenitor cells and faster healing (Muraoka, 2013), and as a control in engineered cardiac tissues (Serrao, 2012).

Application

Injury recovery, connective tissue research, laminin and fibronectin production, extracellular matrix, tissue organization, wound healing, fibroblasts growth factor response.

Biochem/physiol Actions

Skin

Preparation Note

• 1st passage, >500,000 cells in Rat Fibroblast Basal Medium that contains 10% FBS and 10% DMSO
• Can be cultured at least 8 population doublings

Analysis Note

Each lot was tested for proper morphology, Population Doublings, Negative for HIV, Hepatitis B, Hepatitis C, mycoplasma, bacteria, and fungi.

Other Notes

Rat Fibroblast Basal Medium that contains 10% FBS and 10% DMSO