TRAIL, Soluble human，20UG，SRP8024-20UG，Sigma 供应商、价格、厂家、报价、规格参数

产品介绍 Product Description

General description

TNF-related apoptosis-inducing ligand (TRAIL; Apo2L; CD253; TNFSF10) is a type II transmembrane protein of about 34kDa. Like most members of the tumor necrosis factor (TNF) superfamily of cytokines, TRAIL can be cleaved at the cell surface by metalloproteases to form a soluble molecule. The gene encoding TRAIL is localized on human chromosome 3q26.31.

Application

TRAIL, soluble human has been used in cell culture, chemical transfection and in caspase-3/7 activity assay.

Biochem/physiol Actions

Trimerized TNF-related apoptosis-inducing ligand (TRAIL) triggers apoptosis upon ligation of cell surface TRAIL-receptor 1 (R1) and/or TRAIL-R2 by inducing the formation of the so-called multiprotein death-inducing signaling complex (DISC). Active TRAIL forms trimers and specifically binds to five distinct receptors: TRAIL-receptors 1,-2,-3,-4 {TRAIL-R1 (DR4; Apo2; CD261; TNFRSF10A), TRAIL-R2 (DR5; KILLER; TRICK2A; TRICK2B; CD262; TNFRSF10B), TRAIL-R3 (DcR1; LIT; TRID; CD263; TNFRSF10C), TRAIL-R4 (DcR2; TRUNDD; CD264; TNFRSF10D)} and osteoprotegerin (OPG; OCIF; TNFRSF11B).

Physical form

Solution in 20 mM HEPES, pH 7.9, 120 mM NaCl, 0.01% Tween 20, 1% sucrose, 1 mM DTT.

Other Notes

The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a His-tag and a linker peptide. The active multimeric conformation is stabilized by an inserted mutation allowing an additional CC-bridge.

技术参数 Specifications

biological source	human
recombinant	expressed in E. coli
tag	His tagged
assay	>98% (SDS-PAGE)
form	liquid
mol wt	~26 kDa
packaging	pkg of 20 μg
concentration	≥0.2 mg/mL
technique(s)	activity assay: suitable,cell culture \| mammalian: suitable,transfection: suitable
impurities	<0.01 EU/μg endotoxin, tested
color	clear
UniProt accession no.	P50591
shipped in	dry ice
storage temp.	−70°C
Gene Information	human ... TNFSF10(8743)

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