- 商品介绍
- 规格参数
- 包装参数
Application
Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation.
不同种类的马铃薯(S. tuberosum)中存在至少2种同工酶：一种的ATP酶/ADP酶比例较高(∼10)和另一种较低(∼1)。
反应：ATP → ADP+Pi → AMP+2Pi。
Biochem/physiol Actions
Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis. Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.
Physical form
Partially purified, lyophilized powder containing potassium succinate buffer salts.
Preparation Note
按 ATP 酶单位销售。
Other Notes
该等级具有低 ATP 酶/ADP 酶比率。
One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.
| Quality Segment | 400 |
| type | Grade VII |
| form | lyophilized powder |
| ATPase activity | ≥200 units/mg protein |
| secondary activity | ≥50 % of base activity ADPase |
| composition | Protein, 25-60% |
| solubility | H2O: soluble 1.0 mg/mL |
| application(s) | diagnostic assay manufacturing |
| foreign activity | Acid Phosphatase ≤2% of base activity |
| storage temp. | −20°C |
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