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General description
Polypyrimidine tract-binding protein 1 (UniProt: P26599; also known as PTB, 57 kDa RNA-binding protein PPTB-1, Heterogeneous nuclear ribonucleoprotein I, hnRNP I) is encoded by the PTBP1 (also known as PTB) gene (Gene ID: 5725) in human. PTB is a monomeric, sequence-specific, RNA binding protein that is involved in multiple aspects of messenger RNA metabolism, including the repression of alternative exons. It contains four RNA binding domains (RBDs) with each bound to a CUCUCU oligonucleotide. Three linker amino acid sequences separate the RBDs along the polypeptide sequence. Each RBD binds RNA with a different binding specificity. In PTB RBD3 and RBD4 interact, resulting in an antiparallel orientation of their bound RNAs. Hence, PTB induces RNA looping when bound to two separated pyrimidine tracts within the same RNA. PTB plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. It activates exon skipping of its own pre-mRNA during muscle cell differentiation. It is reported to bind to polypyrimidine-rich controlling element (PCE) of CFTR and promote exon skipping of CFTR exon 9, thereby antagonizing TIA1 and its role in exon inclusion of CFTR exon 9. It is reported to be involved in the splicing of pyruvate kinase PKM by binding repressively to a polypyrimidine tract flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform. isoforms of PTB have been described that are produced by alternative splicing. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: David, CJ., et al. (2010). Nature. 463(7279); 364-368; Oberstrass, FC., et al. (2005). Science. 309(5743); 2054-2057; Zuccato, E., et al. (2004). J. Biol. Chem. 279(17); 16980-16988).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 18 amino acids from the N-terminal half of human Polypyrimidine tract-binding protein 1 (PTBP1/PTB).
Application
Quality Control Testing
Evaluated by Western Blotting in NIH3T3 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PTBP1/PTB in NIH3T3 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PTBP1/PTB in L6 cell lysate.
Affinity Binding Assay: A representative lot of this antibody bound PTBP1/PTB peptide with a KD of 6.7 x 10-6 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected PTBP1/PTB in human small intestine tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 1G23 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Polypyrimidine tract-binding protein 1 (PTBP1). It targets an epitope within 18 amino acids from the N-terminal half.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1G23, recombinant monoclonal |
| description | 1G23 Clone |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 59.63 kDa,observed mol wt ~55 kDa |
| purified by | using Protein A |
| species reactivity | human, monkey, rat, mouse |
| species reactivity (predicted by homology) | monkey |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | N-terminal half |
| Protein ID accession no. | NP_114368 |
| UniProt accession no. | P26599 |
| greener alternative category | Aligned, |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... PTBP1(5725) |
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