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General description
Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial (UniProt: Q15118; also known as EC:2.7.11.2, Pyruvate dehydrogenase kinase isoform 1, PDH kinase 1) is encoded by the PDK1 (also known as PDHK1) gene (Gene ID: 5163) in human. PDH kinase 1 is a mitochondrial matrix enzyme that is synthesized with a transit peptide (aa 1-28), which is subsequently cleaved off. It is expressed predominantly in the heart and is detected at lower levels in liver, skeletal muscle, and pancreas. It plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the TCA cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-CoA from pyruvate. It is also reported to play an important role in cellular responses to hypoxia and is shown to be important for cell proliferation under hypoxic conditions. It also protects cells against apoptosis in response to hypoxia and oxidative stress. Its levels are up-regulated via the HIF1 signaling in response to hypoxia. Exposure of cancer cells to severe hypoxia is reported to induce translocation of AKT to the mitochondrion, which leads to AKT-mediated phosphorylation of PDH kinase 1 at threonine 346 that supports tumor cell survival and proliferation during hypoxia. PDH kinase 1 is phosphorylated by constitutively activated Abl1, FGFR1, FLT3 and JAK2. Its phosphorylation at tyrosine 243 and 244 strongly increases its kinase activity, while phosphorylation at tyrosine 136 has reduced effect. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonal. (Ref.: Chae YC., et al. (2016). Cancer Cell. 30(2); 257-272; McFate, T., et al. (2008). J. Biol. Chem. 283(33); 22700-22708; Gudi, R., et al. (1995). J. Biol. Chem. 270(48); 28989-28994).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of human Pyruvate dehydrogenase kinase 1 (PDH kinase 1).
Application
Quality Control Testing
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PDH kinase 1 in HeLa cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PDH kinase 1 in lysates from RAW264.7 and C6 cells.
Affinity Binding Assay: A representative lot of this antibody bound PDH kinase 1 peptide with a KD of 1.0 x 10-12 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected PDH kinase 1 in HeLa cells.
Flow Cytometry Analysis: 1 µg from a representative lot detected PDH kinase 1 in one million Hela cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected PDH kinase 1 in Human cerebral cortex tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 1C7-H1 ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Pyruvate dehydrogenase kinase 1 (PDH kinase 1). It targets an epitope within 15 amino acids from the C-terminal region.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | recombinant monoclonal |
| description | clone 1C7-H1 ,recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 49.24 kDa,observed mol wt ~44 kDa |
| purified by | using Protein A |
| species reactivity | mouse, human, rat |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,flow cytometry: suitable,immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | C-terminal |
| Protein ID accession no. | NP_002601 |
| UniProt accession no. | Q15118 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Gene Information | human ... PDK1(5163) |
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