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General description
This IMDM differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures.
Application
Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco¢s Modified Eagle¢s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies demonstrated that Iscove¢s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.
Legal Information
AQmedia is a trademark of Sigma-Aldrich Co. LLC
| Quality Level | 400 |
| sterility | sterile-filtered,sterile |
| product line | AQmedia™ |
| form | liquid |
| technique(s) | cell culture | mammalian: suitable |
| impurities | Endotoxin, tested |
| components | stable glutamine: yes NaHCO3: yes glucose: yes phenol red: yes |
| shipped in | ambient |
| storage temp. | 2-8°C |
| 长度(mm) | |
| 宽度(mm) | |
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¥ 4897.21
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¥ 3319.60
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