- 商品介绍
- 规格参数
- 包装参数
General description
KiCqStart SYBR Green qPCR ReadyMix是2倍浓缩的即用型预混液，含有除引物和模板之外的实时定量PCR(qPCR)所需的全部组分。这种专有缓冲液、稳定剂和Taq DNA热启动聚合酶的独特组合使用快速或常规的SYBR Green qPCR循环方案，具有**的PCR效率、灵敏度、特异性和稳定可靠的荧光信号。
高特异性扩增对于使用SYBR Green I染料技术成功进行qPCR至关重要，因为该染料可结合扩增过程中产生的任何dsDNA并进行检测。Taq DNA热启动聚合酶在PCR变性步骤开始之前，处于抗体介导的失活状态。
Application
KiCqStart® SYBR® Green qPCR ReadyMix™预混液已用于实时定量PCR反应(qPCR)，用于扩增和定量来自人肠道普雷沃菌(Prevotella copri)的基因组DNA。
PCR扩增：
- 基因表达
- DNA定量
- 染色质免疫沉淀(CHiP)
Features and Benefits
- 只需33分钟即可获得分析结果
- 高效、灵敏的实时PCR结果
- 几乎不需要多少优化，甚至无需优化
Other Notes
2X反应缓冲液含有优化浓度的MgCl2、dNTP(dATP, dCTP, dGTP, dTTP)、KiCqStart Taq DNA聚合酶、SYBR Green染料和稳定剂。
包装：
250次反应* = 2 X 1.25 mL试管
1250次反应* = 10 X 1.25 mL试管
5000次反应* = 1 X 50 mL试管
*设一次反应需20uL
仪器兼容性：
不同的实时PCR系统采用不同策略来校准荧光信号和校正孔间光学变化。 为特定仪器匹配合适的qPCR试剂至关重要。 KiCqStart SYBR Green qPCR ReadyMix不含内参染料。 兼容仪器：
- Bio-Rad® CFX384™
- Bio-Rad CFX96™
- Bio-Rad MiniOpticon™
- Bio-Rad/MJ Chromo4™
- Bio-Rad/MJ Opticon 2
- Bio-Rad/MJ Opticon™
- Cepheid SmartCycler®
- Eppendorf Mastercycler® ep realplex
- Eppendorf Mastercycler ep realplex2 s
- Illumina Eco qPCR
- Qiagen/Corbett Rotor-Gene® 3000
- Qiagen/Corbett Rotor-Gene 6000
- Qiagen/Corbett Rotor-Gene Q
- Roche LightCycler® 480
储存条件：
KiCqStart SYBR Green qPCR ReadyMix在-20°C的恒温冷冻柜中避光保存，可稳定存放1年。为方便使用，也可在+2至+8°C下储存长达6周。解冻后，充分混匀后再使用。不建议反复冻融产品。不过，冻融20次或+20°C存放2个月后，产品没有出现任何性能损失。
Legal Information
LightCycler is a registered trademark of Roche
Mastercycler is a registered trademark of Eppendorf SE
MiniOpticon is a trademark of Bio-Rad Laboratories, Inc.
Opticon is a trademark of Bio-Rad Laboratories, Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
Rotor-Gene is a registered trademark of Qiagen GmbH
SYBR is a registered trademark of Thermo Fisher Scientific or its subsidiaries
SmartCycler is a registered trademark of Cepheid, Inc.
Bio-Rad is a registered trademark of Bio-Rad Laboratories, Inc.
CFX384 is a trademark of Bio-Rad Laboratories, Inc.
CFX96 is a trademark of Bio-Rad Laboratories, Inc.
Chromo4 is a trademark of Bio-Rad Laboratories, Inc.
Eppendorf is a registered trademark of Eppendorf SE
KiCqStart is a registered trademark of QIAGEN Beverly Inc.
| form | liquid |
| usage | sufficient for 1250 reactions, sufficient for 250 reactions, sufficient for 5000 reactions |
| feature | dNTPs included,hotstart |
| storage condition | protect from light |
| technique(s) | RT-qPCR: suitable,qPCR: suitable |
| color | colorless |
| input | purified DNA |
| web metadata keyword.default | microbialanalysis microbialanalysis |
| compatibility | for use with Bio-Rad CFX384,for use with Bio-Rad CFX96,for use with Bio-Rad MJ Chromo4,for use with Bio-Rad MJ Opticon 2,for use with Bio-Rad MJ Opticon Cepheid SmartCycler,for use with Bio-Rad MiniOpticon,for use with Bio-Rad MyiQ,for use with Eppendorf® Mastercycler ep realplex2 s,for use with Eppendorf® Mastercycler ep realplex,for use with Illumina Eco qPCR,for use with Qiagen Corbett Rotor-Gene 3000,for use with Qiagen Corbett Rotor-Gene 6000,for use with Qiagen Corbett Rotor-Gene Q,for use with Roche LightCycler 480 |
| detection method | SYBR® Green |
| shipped in | dry ice |
| storage temp. | −20°C |
| 长度(mm) | |
| 宽度(mm) | |
| 高度(mm) | |
| 重量(kg) |
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