Anti-phospho-RB1 (S807) antibody, clone 1H3, Rabbit Monoclonal，1004G，SAB6010035-100UL，Sigma 供应商、价格、厂家、报价、规格参数

产品介绍 Product Description

General description

Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 ′Lys-20′ trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1′s activity.

Immunogen

A synthesized peptide derived from human Phospho-RB1 (S807)

Physical form

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Preparation Note

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Other Notes

786-828aa

技术参数 Specifications

recombinant	expressed in HEK 293 F cells
Quality Segment	100
conjugate	unconjugated
antibody product type	primary antibodies
clone	1H3, recombinant monoclonal
form	liquid
mol wt	150 kDa
purified by	affinity chromatography
species reactivity	human
concentration	1.2mg/ml
technique(s)	ELISA: 2000:10000,flow cytometry: 1:50-1:200,immunofluorescence: 1:50-1:200,immunohistochemistry: 1:50-1:200
isotype	IgG
UniProt accession no.	P06400
shipped in	wet ice
storage temp.	-10 to -25°C
target post-translational modification	phosphorylation (pSer807)

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