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General description
Serine/threonine-protein phosphatase 2B catalytic subunitgamma isoform (UniProt: P48454; also known as EC:3.1.3.16, CAM-PRP catalyticsubunit, Calcineurin, testis-specific catalytic subunit, Calmodulin-dependentcalcineurin A subunit gamma isoform, PPP3CC) is encoded by the PPP3CC (alsoknown as CALNA3, CNA3) gene (Gene ID: 5533) in human. PPP3CC is acalcium-dependent, calmodulin stimulated protein phosphatase that plays anessential role in the transduction of intracellular Ca2+-mediated signals. It isactivated by Ca2+-bound calmodulin following an increase in intracellular Ca2+.At low Ca2+ concentrations, the catalytic subunit (aa 52-343; calcineurin A) isinactive and is bound to the regulatory subunit (calcineurin B) in which onlytwo high-affinity binding sites are occupied by Ca2+. In response to elevatedcalcium levels, the occupancy of the low-affinity sites on calcineurin B byCa2+ causes a conformational change of the C-terminal regulatory domain ofcalcineurin A, resulting in the exposure of the calmodulin-binding domain (aa386-400) and in the partial activation of calcineurin A. The subsequent bindingof Ca2+-bound calmodulin leads to the displacement of the autoinhibitory domain(aa 451-489) from the active site and possibly of the autoinhibitory segment(aa 401-408) from the substrate binding site which fully activates calcineurinA. This ZooMAb® recombinant monoclonal antibody, generated by our proprietarytechnology, offers significantly enhanced specificity, affinity,reproducibility, and stability over conventional monoclonals.
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Immunogen
KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of human Serine/threonine-protein phosphatase 2B catalytic subunit gamma isoform (PPP3CC).
Application
Anti-PPP3CC, clone 2C2 ZooMAb, Cat. No. ZRB1443, is a recombinant Rabbit monoclonal antibody that specifically targets PPP3CC and is tested in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PPP3CC in Jurkat and human testis lysates.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected PPP3CC in Jurkat and MCF-7 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected PPP3CC in human testis tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound PPP3CC peptide with a KD of 1.1 x 10-7 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biochem/physiol Actions
Clone 2C2 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Serine/threonine-protein phosphatase 2B catalytic subunit gamma isoform (PPP3CC). It targets an epitope within 17 amino acids from the C-terminal region.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in lyophilized from PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit (recombinant) |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 2C2, monoclonal,recombinant monoclonal |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 58.13 kDa,observed mol wt 58 kDa |
| species reactivity | human |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| UniProt accession no. | Q15121 |
| greener alternative category | , Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... PPP3CC(5533) |
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