- 商品介绍
- 规格参数
- 包装参数
General description
Anti-Human IgG (Fab specific)-Peroxidase antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the GG-6 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from mouse immunized with purified human IgG (Fab fragment). IgGs are the most common Immunoglobulins isotype in blood, lymph fluid, cerebrospinal fluid and peritoneal fluid and a key players in the humoral immune response. IgGs include four subclasses (IgG1, IgG2, IgG3, and IgG4), they consist of a variable Fab fragment (which includes the antibody recognition site), and a conserved Fc fragment. The IgG subclasses differ in their physical and chemical properties, their distribution pattern is found to be age-dependent, and every subclass has a specific biological function.
Immunogen
Purified human IgG (Fab Fragment)
Application
Direct ELISA: a working dilution of 1:16,000-1:32,000 is recommended using 2.5 μg/mL human IgG for coating.
Biochem/physiol Actions
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. Maternal IgG is the only antibody transported across the placenta to the fetus. Maternal IgG passively immunizes the infants. IgG deficiencies are often associated with various diseases.
Physical form
Supplied as a lyophilized powder.
Other Notes
In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.
| biological source | mouse |
| Quality Level | 200 |
| conjugate | peroxidase conjugate |
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| clone | GG-6, monoclonal |
| form | lyophilized powder |
| species reactivity | human |
| concentration | ~2 mg/mL |
| technique(s) | direct ELISA: 1:16,000-1:32,000 using 2.5 μg/ml human IgG for coating. |
| isotype | IgG1 |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| 长度(mm) | |
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