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General description
Histone-lysine N-methyltransferase 2D (UniProt O14686; also known as EC 2.1.1.43, ALL1-related protein, Lysine N-methyltransferase 2D, Myeloid/lymphoid or mixed-lineage leukemia protein 2, MLL2) is encoded by the KMT2D (also known as ALR, KABUK1, MLL2, MLL4) gene (Gene ID: 8085) in human. MLL2 is a histone methyltransferase that catalyzes methyl group transfer from S-adenosyl-L-methionine to the -amino group of lysine 4 of histone H3 (H3K4) that represents a specific tag for epigenetic transcriptional activation. Its expression is observed in most adult tissues, including a variety of hematopoietic cells. However, it has not been detected in the liver. It predominantly forms H3K4me1 methylation marks at active chromatin sites where transcription and DNA repair take place. It forms a core complex with the evolutionary conserved subcomplex WRAD that is composed of WDR5, RBBP5, ASH2L/ASH2 and DPY30 subunits. Mutations in KMT2D gene have been linked to Kabuki syndrome 1, a congenital mental disorder with multiple abnormalities, including dwarfism, scoliosis, and radiographic abnormalities of the vertebrae, hands, and hip joints. Inactivating mutations in MLL2 gene have been linked to medulloblastoma. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Shinsky, SA., et al. (2015). J. Biol. Chem. 290(10); 6361-6375; Ng, SB., et al. (2010). Nat. Genet. 42(9); 790-793; Parsons, D.W., et al. (2011). Science 331, 435-439).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 22 amino acids from the C-terminal region of human Myeloid/lymphoid or mixed-lineage leukemia protein 2 (MLL2).
Application
Quality Control Testing
Evaluated by Immunohistochemistry (Paraffin) in human placenta tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution of this antibody detected MLL2 in human placenta tissue sections.
Tested Applications
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MLL2 in U2OS cells.
Affinity Binding Assay: A representative lot of this antibody bound MLL2 C-terminal peptide with a KD of 3.8 x 10-8 in an affinity binding assay.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MLL2 in MOLM13 and HEK293 cell lysates. (Courtesy of Dr Shu-Ping Wang, Institute of Biomedical Sciences, Academia Sinica, Taiwan).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biochem/physiol Actions
Clone 3L17 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Myeloid/lymphoid or mixed-lineage leukemia protein 2 (MLL2). It targets an epitope within 22 amino acids from the C-terminal region.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 3L17, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 593.39 kDa |
| purified by | using Protein A |
| species reactivity | human |
| species reactivity (predicted by homology) | bovine, mouse, zebrafish, feline, horse, canine, monkey |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | C-terminal |
| Protein ID accession no. | NP_003473 |
| UniProt accession no. | O14686 |
| greener alternative category | Aligned, |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... KMT2D(8085) |
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