桑格全基因组 慢病毒 CRISPR 文库，，MSANGERV-1EA，Sigma

General description

两名基因组编辑领导者Sigma-Aldrich和Wellcome Trust Sanger Institute联手打造了**个阵列化的慢病毒CRISPR基因敲除文库。基于文献中已验证的技术，Sanger CRISPR库将使您的实验室走在竞争的最前沿，以实现下一个重大发现。

Application

功能基因组学/筛选/靶标验证

Features and Benefits

• Vector: U6-gRNA/PGK-Puro-2A-BFP (gRNA only)
• Simplify the workflow with puromycin selection
• Illuminate CRISPR-expressing cells with BFP

• Better, not bigger: Two optimized clones per mouse gene reduces the time, cost, and scale of screening experiments
• Ready-to-screen: Clones are arrayed in a robotics-friendly 384-well format for high throughput screening
• Collaborative: Real-time, library validation continues

Packaging

384 well plates

Physical form

10μl of lentivirus at ≥10⁶ particles/ml in ~120×384 well plates

Other Notes

Additional libraries are available:
Human, Glycerol Format
Mouse, Glycerol Format
Human, Virus Format
Lentivirus Transduction Particles of gRNA-only lenti-based vectors. 2 knockout clones for every human protein-coding gene. Nearly 40,000 sequence confirmed clones


Request a Quote or More Information
This product is for R&D use only, not for drug, household, or other uses. Lentivirus manufacturing is achieved by using 2nd generation packaging plasmids on a 3rd generation lenti-based vector. Though the lentiviral transduction particles produced are replication incompetent, it is recommended that they be treated as Risk Group Level 2 (RGL-2) organisms in laboratory handling. Follow all published RGL-2 guidelines for laboratory handling and waste decontamination.

Legal Information

CRISPR Use License Agreement

Lentiviral License Agreement