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General description
Monoclonal Anti-Cathepsin B specifically recognizes human1 and rat1 cathepsin B (~ 25 kDa). The antibody epitope resides within the peptide sequence EPGYSP which corresponds to amino acids 212-217 of human cathepsin B. The antibody does not cross react with the closely related lysosomal cysteine proteases: cathepsins L, H, K, S, V, and W
The CTSB (Cathepsin B) gene encoding cathepsin B is mapped to human chromosome 8p23.1. Cathepsin B are normally localized in lysosomes of almost all mammalian cells, but under certain conditions they can be secreted from the cell that take part in local proteolysis. Cathepsin B is both an endo- and exopeptidase, composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor.
Immunogen
Recombinant human cathepsin B.
Application
indirect ELISA, immunohistochemistry, In HCT-116 cells. 4-8μg/mL of antibody concentration may be used. A dilution of 1:800 in human pancreatic carcinoma cell line PANC-1 was used for immunoblotting
Biochem/physiol Actions
Cathepsins are lysosomal proteases activation of enzyme precursors, and tumor invasion and metastasis. It is involved in the proteolysis of many proteins involved in different physiological and pathological pathways. In the brain, this protease, also known as amyloid precursor protein secretase, is responsible for the proteolytic processing of amyloid precursor protein (APP). An incomplete proteolytic processing of APP has been found as a causative factor in Alzheimer disease. Furthermore, ablated genes coding cathepsin B and L leads to enormous neuronal cell death,probably due to autophagy abnormalities. Cathepsin B has been implicated in tumor progression by virtue of localization at the invading front of the tumor. For example, blocking cathepsin B expression in human glioblastoma SNB19 cells by a stable transfection of an expression vector expressing antisense cathepsin B, caused suppression of glioblastoma-induced neovascularization.
Monoclonal Anti-Cathepsin B specifically recognizes human1 and rat1 cathepsin B (~ 25 kDa). The antibody epitope resides within the peptide sequence EPGYSP which corresponds to amino acids 212-217 of human cathepsin B. The antibody does not cross react with the closely related lysosomal cysteine proteases: cathepsins L, H, K, S, V, and W.
Cathepsins significantly takes part in the immune response, by processing antigens into peptides for its presentation through MHC class II molecules.
Physical form
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 200 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | CB59-4B11, monoclonal |
| form | buffered aqueous solution |
| mol wt | antigen ~25 kDa |
| species reactivity | rat, human |
| concentration | ~1.5 mg/mL |
| technique(s) | immunohistochemistry: suitable,indirect ELISA: suitable,western blot: 4-8 μg/mL using HCT-116 cells. |
| isotype | IgG1 |
| UniProt accession no. | P07858 |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... CTSB(1508) rat ... CTSB(64529) |
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