Monoclonal Anti-Bovine IgG antibody produced in mouse,0.2ML,B6901-.2ML,Sigma

销售价: ¥ 2120.59 / 件
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订货号 0BT5649
品牌型号 Sigma B6901-.2ML
货期 询货期
最小订货量 1件
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产品介绍 Product Description

General description

The antibody reacts with an epitope on both bovine IgG1 and IgG2 in their native form. The antibody shows no reaction with the epitope after denaturation and reduction. IgG (immunoglobulin G) contributes to 10–20% of plasma protein and is regarded as one of the most predominant serum protein. It consists of four subclasses: IgG1, IgG2, IgG3 and IgG4. The IgG structure possesses four polypeptide chains containing two identical γ heavy (H) chains and two identical γ or γ light (L) chains of 50 kDa and 25 kDa, respectively.


Immunogen

Purified bovine IgG


Application

Monoclonal Anti-Bovine IgG antibody has been used in indirect immunoperoxidase staining.
Monoclonal Anti-Bovine IgG antibody produced in mouse is suitable for ELISA at a working dilution of 1:20,000. It may be used for immunohistochemistry of formalin-fixed, paraffin-embedded sections at a dilution of 1:1000. Monoclonal Anti-Bovine IgG antibody produced in mouse was used as an isotype control in ChIP assay.


Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.
bovine IgG1 and IgG2


Physical form

Ascites fluid containing 0.1% sodium azide


Preparation Note

For continuous use, store at 2-8°C. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.


Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


技术参数 Specifications
biological sourcemouse
Quality Level200
conjugateunconjugated
antibody formascites fluid
antibody product typesecondary antibodies
cloneBG-18, monoclonal
contains15 mM sodium azide
species reactivitybovine (non-reactive to IgM)
should not react with pig, human, rabbit, sheep, cat, guinea pig, canine, horse, goat
technique(s)dot blot: suitable,indirect ELISA: 1:1000
isotypeIgG1
shipped indry ice
storage temp.−20°C
target post-translational modificationunmodified
长度(mm)
宽度(mm)
高度(mm)
重量(kg)
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