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General description
Tumor necrosis factor receptor 1 (TNFR1), also known as tumor necrosis factor receptor superfamily member 1A (TNFRSF1A) and CD120a (cluste of differentiation 120a), is an ubiquitous membrane receptor that binds tumor necrosis factor-alpha (TNFα). It has been shown to be localized to both plasma membrane lipid rafts and the trans golgi complex with the help of the death domain (DD). TNFR1 can activate the transcription factor NF-κB, mediate apoptosis, and regulate inflammation processes.Alpaca derived anti-TNFR1 VHH single domain antibody (molecular weight: 14.2 kDa), expressed in E. coli under conditions free from animal derived components.It has a 6His tag at its C-terminal.
Single-domain antibody (sdAb), also called nano-antibody, is the variable region isolated from the antigen-binding variable domain (VHH) of camelid heavy-chain-only antibody. It lacks light chains and a first constant domain(CH1). This configuration confers a number of unique advantages over conventional monoclonal antibodies. The small size of just 15 kDa (2~4 nm), single-domain antibody is the smallest antigen-binding fragment that can easily penetrate tissues, allowing them to bind epitopes that are inacessible to conventional antibodies. These single-domain antibodies offer superior chemical and thermal stability and increased solubility than conventional antibodies. All our antibodies are tested to ensure the highest level of specificity, sensitivity and reproducibility.
Immunogen
Recombinant human TNFR1 (Ile22-Thr211) protein
Application
ELISA, WB, IHC, IF
Biochem/physiol Actions
Human Tumor necrosis factor receptor 1 (TNFR1) / CD120a
Packaging
50 µg per vial / 100 µg per vial
Physical form
Affinity purified VHH single domain antibody lyophilized from PBS, pH7.6
Preparation Note
Reconstitute the lyophilized antibody with sterile deionized water or with 50% glycerol (ACS grade or better) to a stock solution of 1 mg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.
Stable for 1 year at 4°C from date of receipt.
Other Notes
Storage at 4°C; Do not freeze.
| biological source | alpaca |
| Quality Segment | 100 |
| recombinant | expressed in E. coli |
| conjugate | unconjugated |
| antibody form | affinity purified VHH antibody |
| clone | TR09, recombinant monoclonal |
| assay | >95% (SDS-PAGE) |
| form | lyophilized solid |
| mol wt | 14.2 kDa, ~15-18 kDa (SDS PAGE under reducing condition) |
| purified by | immobilized metal affinity chromatography (IMAC) |
| species reactivity | human |
| concentration | 1 mg/mL |
| technique(s) | ELISA: 1:1000-1:10000,immunofluorescence: 1:100-1:300,immunohistochemistry: 1:100-1:300,western blot: 1:500-1:3000 |
| color | colorless |
| pH | ( in PBS, pH 7.4) |
| isotype | VHH |
| Protein ID accession no. | NP_001056.1 |
| UniProt accession no. | P19438 |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... TNFRSF1A(7132) |
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¥ 1451.54
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¥ 3335.66
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¥ 2307.00
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¥ 165230.53
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