Anti-E2F3 antibody ,Mouse monoclonal，0.2ML，E8651-.2ML，Sigma

General description

Monoclonal Anti-E2F3 (mouse IgG2a isotype) is derived from the PG30 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant human E2F Transcription Factor 3 (E2F3) protein. Transcription factor E2F3 is a DNA-binding protein of ~57 kDa encoded by the E2F3 gene in humans. It belongs to E2F transcription factor family of proteins that bind to the sequence TTTCGCGC.

Immunogen

recombinant human E2F3.

Application

Monoclonal Anti-E2F3 antibody produced in mouse has been used for the following applications:

• immunoprecipitation using denatured E2F3
• western blotting at a concentration of 4-8μg/mL using transfected 293T cells expressing E2F3
• electrophoretic mobility shift assay (EMSA).

Biochem/physiol Actions

Transcription factor E2F3 is involved in the regulation of cell cycle. E2F3 helps in the regulation of the expression of a number of genes that are important in cell proliferation. It is a oncogene with strong proliferative potential and is involved in many processes. E2F3 plays an important role in the development of several types of cancer. Overexpression of E2F3 may be associated with hepatocellular carcinoma (HCC). It plays an important role in controlling cell cycle progression and proliferation. E2F3 is frequently expressed in pediatric Wilms′ tumors and may be associated with the same.

Physical form

Solution in phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Preparation Note

Purified from culture supernatant of hybridoma cells grown in a bioreactor.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.