Red Protease Detection Kit，1KIT，CS0013-1KT，Sigma

General description

The Red Protease Detection Kit uses TAMRA-casein as a convenient fluorescent substrate for detection of proteases in various samples.

Fluorescence excitation wavelength = 535 nm

Fluorescence emission wavelength = 595 nm

Application

Detection and quantitation of protease activity in samples. Useful for impurity testing.
Detection limit approximately 19 ng/ml, when using a 1 hour incubation with Trypsin as a standard.

Features and Benefits

• Fluorescence is pH-independent
• No separation or precipation steps are required
• High sensitivity and signal-to-noise
  

Other Notes

CS0013A Trypsin (1 mg/mL): 100 μL

CS0013B Protease AssayBuffer × 2: 30 mL

CS0013C TAMRA-Casein substrate: 280 μL