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General description
Ras-related protein Rab-5A (UniProt: P20+D9339; also known as EC:3.6.5.2, Rab5A) is encoded by the RAB5A (also known as RAB5) gene (Gene ID: 5868) in human. Rab5A is a member of the small GTPase superfamily that is localized in the cytosolic side of plasma membrane and endosomes. It plays important roles in cellular differentiation, neurogenesis, exocytosis, and protein transport. It is a rate-limiting catalyst of the endocytic uptake and delivery to early endosomes of both fluid-phase and receptor-mediated endocytic tracers, as well as of homotypic endosomal fusion. It is required for endosome fusion with the plasma membrane. It localizes to early endosomes where it is involved in the recruitment of RAB7A and the maturation of these compartments to late endosomes. It drives the maturation of endosomes by transporting vacuolar (H+)-ATPases from trans-Golgi network to endocytic vesicles. Rab5A cycles between active GTP-bound and inactive GDP-bound states. In its active state, it binds to a variety of effector proteins to regulate cellular responses such as of intracellular membrane trafficking and from the formation of transport vesicles and their fusion with membranes. Active GTP-bound form recruits different sets of downstream effectors to membrane that are directly responsible for vesicle formation, movement, tethering, and fusion. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Croizet-Berger, K., et al. (2002). Proc. Natl. Acad. Sci. USA. 99 (12); 8277-8282; Hoffenberg, S., et al. (2000). J. Biol. Chem. 275(32); 24661-24669).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal region of human Ras-related protein Rab5A.
Application
Quality Control Testing
Evaluated by Western Blotting in Neuro2a cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Rab5A in Neuro2a cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Rab5A in lysates from A431, MCF7, PC12 cells and Rat brain tissue.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Rab5A in MCF-7 cells.
Affinity Binding Assay: A representative lot of this antibody bound Rab5 with a KD of 5.1 x 10-6 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biochem/physiol Actions
Clone 3L17 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Ras-related protein Rab5A. It targets an epitope within 16 amino acids from the C-terminal region.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 3L17, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 23.66 kDa,observed mol wt ~25 kDa |
| purified by | using Protein A |
| species reactivity | human, rat, mouse |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunocytochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | C-terminal |
| Protein ID accession no. | NP_004153 |
| UniProt accession no. | P20339 |
| greener alternative category | Aligned, |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... RAB5A(5868) |
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