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General description
Aquaporins function in the movement of water and small solutes across the cell membrane. Aquaporins are widely distributed from bacteria to plants and animals. In E. coli, one aquaporin has been identified that is named AQP-Z. In the plant Arabidopsis thaliana, over 9 AQPs have been characterized. In mammals, at least 11 different AQPs have been cloned. AQP3 provides kidney medullary collecting ducts with high permeability to water, permitting water to move in the direction of an osmotic gradient and may play an important role in gastrointestinal tract water transport. AQP3 and AQP4 are located at the basolateral part of epithelial cells in kidney, colon and trachea.
Rabbit polyclonal anti-Water Channel Aquaporin 3 recognizes rat and mouse aquaporin 3 transport proteins.
Immunogen
synthetic peptide corresponding to amino acids 275-292 of rat aquaporin 3 (with additional N-terminal lysine and tyrosine) conjugated to KLH with glutaraldehyde
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Rabbit anti-AQP3 antibody was used in western blot analysis to identify the level of aquaporin 3 in E. solidaginis larvae. Anti-AQP3 was diluted 1:200 in 5% nonfat dry milk and incubated with the membrane for 2hrs at 21 degrees C.
Rabbit polyclonal anti-Water Channel Aquaporin 3 is used to tag aquaporin 3 for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of aquaporin 3 in kidney medullary collecting ducts and the basolateral surfaces of epithelial cells involved in water transport.
Physical form
Lyophilized from a solution containing phosphate buffered saline, pH 7.4, 1% BSA, and 0.05% sodium azide
Preparation Note
Affinity isolated on immobilized Protein A
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | rat, mouse |
| technique(s) | immunohistochemistry: suitable using Mouse kidney sections,western blot: 1:200 using Rat kidney membranes |
| UniProt accession no. | P47862 |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | mouse ... Aqp3(11828) rat ... Aqp3(65133) |
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