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General description
Prokineticin receptor 2 (UniProt: Q8NFJ6; also known as PK-R2, PKR2, G-protein coupled receptor 73-like 1, G-protein coupled receptor I5E, GPR73b, GPRg2) is encoded by the PROKR2 (also known as GPR73L1, PKR2) gene (Gene ID: 128674) in human. Prokineticins bind to two different G protein-coupled receptors known as prokineticin receptor-1 and -2 (PROKR1 and PROKR2) that display about 85% sequence identity. They bind to PROK1 and PROK2 with nanomolar range affinities. PROKR2 is a multi-pass membrane protein with seven transmembrane domains. Its expression is observed in the ileocecum, thyroid gland, pituitary gland, salivary gland, adrenal gland, testis, ovary, and the brain. It is exclusively coupled to the G(q) subclass of heteromeric G proteins. Its activation leads to mobilization of calcium, stimulation of phosphoinositide turnover, and activation of p44/p42 mitogen-activated protein kinase. It is involved in normal development of the olfactory bulb and sexual maturation. Mice lackin
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal, first extracellular domain of human Prokineticin receptor 2 (PROKR2).
Application
Quality Control Testing
Evaluated by Western Blotting in HeLa cell membrane extract.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PROKR2 in HeLa cell membrane extract.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PROKR2 in A431 and HeLa cell lysates.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected PROKR2 in THP-1 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected PROKR2 in Human cerebral cortex tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 1J6 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Prokineticin receptor 2 (PROKR2). It targets an epitope within 16 amino acids from the first extracellular domain in the N-terminal region.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1J6, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 44 kDa,observed mol wt ~42 kDa |
| purified by | using Protein A |
| species reactivity | human |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | N-terminal |
| Protein ID accession no. | NP_658986.1 |
| UniProt accession no. | Q8NFJ6 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Gene Information | human ... PROKR2(128674) |
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