CRISPR Activator Human Oct4 Positive Control Plasmid，1EA，CRISPR17-1EA，Sigma

General description

OCT4 is the key transcription factor (TF) that maintains pluripotency of embryonic stem cells and plays a critical role in reinstating cellular pluripotency. The expression of OCT4 is stringently silenced in differentiated cells. Activation of silenced OCT4 gene has become a hallmark event during epigenetic reprogramming into iPSCs. The positive control OCT4 gRNA design has been tested with the dCas9-p300 plasmid in HEK293 cells and shown to increase OCT4 mRNA transcript levels over three-fold compared to a negative control.

Application

Functional Genomics/Target Validation

• Epigenetic Modification
• Transcriptional Activation

Features and Benefits

Serves as an experimental control for the CRISPR dCas9-p300 activation system. A positive experiment will result in an increased level of mRNA via qPCR or TaqMan PCR assay.

Other Notes

1 vial containing 1ug of U6-gRNA plasmid expressing a guide sequence to human OCT4 (Octamer-binding transcription factor-4 or POU5F1).
Must be used with dCas9-p300 or other dCas9 activator/repressor plasmids.
To order U6-gRNA plasmids, please visit sigma.com/crisprs

Click here for more information on dcas9-p300 Gene Activator Expression Plasmid.

Legal Information

CRISPR Use License Agreement

Lentiviral and WPRE License Agreements