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General description
T-lymphocyte activation antigen CD86 (UniProt: P42081; also known as Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1) is encoded by the CD86 (also known as CD28LG2) gene (Gene ID: 942) in human. CD86 is a type I transmembrane protein belonging to the immunoglobulin superfamily, containing an extracellular Ig-like V-type (aa 33-131) and Ig-like C2-type (aa 15-225) domains essential for T cell interaction, a transmembrane domain (aa 248-268) and a cytoplasmic domain. The extracellular domain of CD86 is responsible for binding to CD28 or CTLA-4 on T cells. CD86 is expressed on the surface of antigen-presenting cells, including dendritic cells, macrophages, and B cells. Its expression is upregulated upon activation of these cells, which typically occurs during an immune response to pathogens. Its dysregulation has been implicated in autoimmune diseases such as rheumatoid arthritis and multiple sclerosis, allergic reactions, and transplant rejection, making it a potential target for immunotherapy. In some cancers, the manipulation of CD86 and its pathways is explored for therapeutic cancer vaccines.
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 16 amino acids from the extracellular domain of human CD86.
Application
Quality Control Testing
Evaluated by Western Blotting with MCF-7.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected CD86 in MCF-7 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected CD86 in human spleen tissue lysate and a 1:50,000 dilution detected T7/HIS-tagged, human recombinant CD86.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected CD86 in human tonsil tissue sections.
Flow Cytometry Analysis: 1 µg from a representative lot detected CD86 in one million human peripheral blood mononuclear cells (PBMC).
Affinity Binding Assay: A representative lot of this antibody bound CD86 peptide with at least one thousand-fold (1,000X) higher than with non-specific control peptide.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CD86 in HDLM-2 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 2H2 is a ZooMAb® rabbit recombinant monoclonal antibody that detects CD86. It targets an epitope within 16 amino acids from the extracellular domain.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 2H2, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 38 kDa,observed mol wt ~55 kDa |
| purified by | using Protein A |
| species reactivity | human |
| species reactivity (predicted by homology) | monkey |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,flow cytometry: suitable,immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgGκ |
| epitope sequence | Extracellular domain |
| Protein ID accession no. | NP_787058.4 |
| UniProt accession no. | P42081 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Gene Information | human ... CD86(942) |
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