β-羟丁酸脱氢酶 来源于球形红假单胞菌,25UNITS,H8509-25UN,Sigma

销售价: ¥ 14270.65 / 件
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订货号 0BR7473
品牌型号 Sigma H8509-25UN
货期 询货期
最小订货量 1件
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产品介绍 Product Description

Application

适用于通过 Williamson, D. H., and Mellanby, J., Methods of Enzymatic Analysis, Bergmeyer, H., ed., 2nd edition, 4, 1836 (1974) 中介绍方法的测定乙酰乙酸酯和 D(-)-3-羟基丁酸酯。


Biochem/physiol Actions

In mammalian systems, β-hydroxybutyrate dehydrogenase is localized on the inner mitochondrial membrane and requires phosphatidyl choline for activity. In contrast, the enzyme from Rhodopseudomonas is a soluble cytosolic enzyme that does not require a phospholipid allosteric activator. The enzyme is required for the utilization of ketone bodies as a source of metabolic energy. It catalyzes the oxidation of 3-hydroxybutyrate to acetoacetate, the first step in the conversion of ketone bodies to citric acid, which is then further metabolized via the tricarboxylic acid cycle (Krebs cycle).


Physical form

含 Tris 缓冲盐的冻干粉


Other Notes

One unit will oxidize 1.0 μmole of D-β-hydroxybutyrate to acetoacetate per min at pH 7.8 at 37 °C.


技术参数 Specifications
typeType V
Quality Segment200
formlyophilized powder
specific activity250-750 units/mg protein
purified bychromatography
foreign activitylactate dehydrogenase ≤0.05%,malate dehydrogenase ≤0.1%
storage temp.−20°C
长度(mm)
宽度(mm)
高度(mm)
重量(kg)
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