Carboxy-terminal FLAG-BAP™ Fusion Protein,0.1MG,P7457-.1MG,Sigma

销售价: ¥ 3115.61 / 件
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订货号 0BR6946
品牌型号 Sigma P7457-.1MG
货期 询货期
最小订货量 1件
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产品介绍 Product Description

General description

Carboxy-terminal FLAG-BAP Fusion Protein is a 466 amino acid C-terminal FLAG fusion protein of E.coli bacterial alkaline phosphatase (BAP).


Application

Carboxy-terminal FLAG-BAP Fusion Protein has been used in the immunoprecipitation of the reporter protein in human embryonic kidney (HEK) cell lysate and as a FLAG-tagged control protein in solid-phase binding assay of spermatogenic immunoglobulin superfamily protein (SgIGSF).
Learn more product details in our FLAG® application portal.


Biochem/physiol Actions

The FLAG sequence comprises of the eight-amino acid sequence AspTyrLysAspAspAspAspLys and is hydrophilic. FLAG fusion proteins are expressed in bacterial, yeast and mammalian cells. FLAG epitope-tagged bacterial alkaline phosphatase is employed in immunoaffinity purification. Alkaline phosphatase based fusion protein have wide clinical applications in immunodetection, enzyme immunoassay and enzyme-linked immunosorbent assay.


Physical form

Supplied in 10 mM Tris, 120 mM NaCl, 0.05 mM ZnCl2


Preparation Note

Dilute the ANTI-FLAG M2 antibody solution to 10 mg/ml


Other Notes

Control protein


Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG-BAP is a trademark of Sigma-Aldrich Co. LLC


技术参数 Specifications
formliquid
Quality Segment200
mol wt ~49 kDa
shipped indry ice
storage temp.−20°C
长度(mm)
宽度(mm)
高度(mm)
重量(kg)
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