KAPA Taq PCR 试剂盒，500UNITS，KK1022，Roche

General description

KAPA Taq is supplied in a 2X ReadyMix^™ format. It comprises all the components required for PCR except primers and template. Simply use PCR-grade water to make up the required reaction volume. KAPA Taq DNA Polymerase comprises two reaction buffers (Buffer A and Buffer B) or a single buffer with loading dye. It aids convenient direct analysis of the PCR product by agarose gel electrophoresis after cycling. KAPA Taq Buffer A (and KAPA Taq Buffer with dye) are standard Tris-ammonium sulfate-based buffers. KAPA Taq Buffer B is a Tris-potassium chloride buffer. KAPA Taq DNA Polymerase combines with any standard Taq buffer with a pH of 8.3 or higher.

Application

KAPA Taq PCR Kit may be used in:

• High throughput PCR
• Amplification of low copy DNA templates
• Multiplex PCR
• Specific amplification of complex templates
• RT-PCR
• Polymorphism genotyping

Biochem/physiol Actions

KAPA Taq DNA Polymerase is a single-subunit, wild-type Taq DNA polymerase isolated from the thermophilic bacterium Thermus aquaticus. KAPA Taq and KAPA Taq HotStart DNA Polymerase have 5′→3′ polymerase and 5′→3′ exonuclease activities. It does not have 3′ → 5′ exonuclease (proofreading) activity. The enzyme has an error rate of approximately 1 error per 2.2 x 10⁵ nucleotides incorporated. In the hot start formulation, the KAPA Taq associates with a proprietary antibody and inactivates the enzyme until the first denaturation step. Hence, eliminating spurious amplification products and increasing reaction efficiency and sensitivity.

Features and Benefits

High performance :

• Improved sensitivity, specificity and yields
• Novel buffer formulation facilitates specific primer annealing, leading to higher yield of specific product.

• KAPA Taq DNA Polymerase can replace any commercial Taq DNA polymerase in an existing protocol.
• The final MgCl₂ concentration may need to be optimized to account for differences in buffer formulation.
• KAPA Taq Buffers contain MgCl₂ at a final concentration of 1.5 mM. Buffer A is recommended as first approach and for applications requiring high yields.Buffer B is recommended for applications where high sensitivity is required (e.g. when the template is limiting). Both buffers may be evaluated to determine the buffer most suitable for a specific application.
• The KAPA Taq PCR system is suitable for the amplification of fragments up to 3.5 kb from genomic DNA or 5 kb from less complex targets.

Preparation Note

Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long term storage.

Analysis Note

Each batch of KAPA Taq DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230Assay). KAPA Taq Ready Mixes are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination levels.

Other Notes

For Research Use Only. Not for use in diagnostic procedures.

Legal Information

ReadyMix is a trademark of Sigma-Aldrich Co. LLC