ExtrAvidin® magnetic beads，5ML，E2642-5ML，Sigma

General description

ExtrAvidin^® Magnetic beads consist of a de-glycosylated, affinity-purified egg white avidin which is a tetrameric protein with four biotin-binding sites. This protein is conjugated into cyanogen bromide-activated magnetic beads at a ratio of ~4 mg/mL protein-to-beads. [TM= "ExtrAvidin"] magnetic beads are used for the purification of biotinylated macromolecules and complexes. It exploits the high binding affinity of avidin (one of the strongest non-covalent interactions known) while offering the low non-specific background binding typically associated with streptavidin produced by Streptomyces avidinii.
ExtrAvidin magnetic beads.

Application

The ExtrAvidin^® magnetic beads may be used for:

• purification and enrichment of biotinylated macromolecules and complexes, including biotinylated proteins and biotin-tagged fusion proteins.
• immunoprecipitation and immunoaffinity purification.

Features and Benefits

• ExtrAvidin^® Magnetic beads provide high affinity and high specificity binding to biotin (Vitamin B7), including biotinylated proteins and biotin-tagged fusion proteins.
• It provides high specificity binding to biotin with low background, offering a significant advantage over standard avidin or streptavidin-based systems exploits the avidin-biotin interaction (Kd = 10^-15 M), one of the strongest non-covalent interactions known in nature.
• It also provides a high binding capacity of at least 650 nmoles of biotin per 1 mL of settled resin.

Legal Information

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany