Creatinine Deiminase microbial，250UNITS，C9409-250UN，Sigma

General description

Isoelectric point : 4.4
Michaelis constant : 3.5 x 10‾³M (Creatinine)
Structure : 6 subunits per mol of enzyme
Inhibitors : Ag⁺,Hg⁺⁺, o-phenanthroline,monoiodoacetate
Optimum pH : 8.5 – 9.5
Optimum temperature : 65 – 75°C
pH Stability : pH 7.0 – 11.0 (30°C, 20hr)
Thermal stability : Below 65°C (pH 7.5, 1hr)

Application

Creatinine Deiminase microbial has been used:

• to immobilize aminosilylated glass beads based biosensor for ammonia/ammonium and creatinine detection in urine
• in creating creatinine-sensing membrane for biophysical studies
• to investigate the bioelectronic tongue for the simultaneous determination of urea, creatinine and alkaline ions in clinical samples

Biochem/physiol Actions

Creatinine deiminase catalyzes the hydrolysis of creatinine to methylhydantoine and ammonia.

Physical form

Lyophilized powder containing mannitol as stabilizer

Other Notes

One unit will hydrolyze 1.0 μmole of creatinine to N-methylhydantoin and NH₃ per min at pH 7.5 at 37 °C in a coupled system with L-glutamic dehydrogenase.