甘露醇脱氢酶 来源于肠系膜明串珠菌,5UNITS,M9532-5UN,Sigma

销售价: ¥ 2192.00 / 件
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订货号 0BQ6234
品牌型号 Sigma M9532-5UN
货期 询货期
最小订货量 1件
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产品介绍 Product Description

General description

Mannitol dehydrogenase (MDH) is a NAD-dependent enzyme, obtained from several organisms like, Lactobacillus brevis, Leuconostoc mesenteroides, Absidia glauca and Rhodococcus. MDH obtained from L. mesenteroides exhibits similarity in sequence with the medium-chain dehydrogenase/reductase protein family. It also contains four identical subunits of about 38 kDa in size.
Mannitol dehydrogenase is a cytoplasmic enzyme whose primary role is the regulation of intracellular levels of the sugar alcohol mannitol in plants. It catalyzes the pyridine nucleotide dependent reduction of fructose to mannitol.
The molecular weight of mannitol dehydrogenase is 136 kDa.


Application

Can be used for the production of D-mannitol.
It has been used in a study to assess glucosylglycerol and glucosylglycerate as enzyme stabilizers.
This preparation is useful in the determination of mannitol in urine.


Biochem/physiol Actions

Mannitol dehydrogenase (MDH) helps to catalyze the pyridine nucleotide-dependent oxidation of D-mannitol to D-fructose. Mannitol dehydrogenase (MDH) obtained from L. mesenteroides is highly specific for the conversion fructose and mannitol.


Physical form

含缓冲盐、磷酸钾以及二硫苏糖醇的冻干粉


Preparation Note

部分纯化


Analysis Note

根据 Yamanaka, Meth. Enzymol., 41(B), 138 (1975) 所述方法进行分析。


Other Notes

在 pH 5.3 和 30°C 且存在 NADH 的条件下,一单位本品每分钟将还原 1.0μmol D-果糖。如上定义的一单位酶相当于如 Lunn 等人所述的约 1.8 单位酶,并且在 pH 8.6 和 40°C 且存在 NAD 的条件下,一单位酶每分钟将氧化 1.0μmol D-甘露醇。


技术参数 Specifications
formlyophilized powder
Quality Segment200
specific activity≥60 units/mg protein
mol wt 136 kDa
storage temp.−20°C
长度(mm)
宽度(mm)
高度(mm)
重量(kg)
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