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General description
Caspase-7 (UniProt: P55210; also known as EC:3.4.22.60, CASP-7, Apoptotic protease Mch-3, CMH-1, ICE-like apoptotic protease 3, ICE-LAP3) is encoded by the CASP7 (also known as MCH3) gene (Gene ID: 840) in human. Activation of caspases is one of the most widely recognized features of apoptosis. Caspases are secreted as latent single chain zymogens, which are further processed to create active caspases that initiate the death program by destroying key components of the cellular infrastructure and activating factors that mediate damage to the cells. An important feature of caspases is that they are not associated with any nonspecific degradative process but are activated only in response to specific signaling event. Caspase-7, a member of the executioner group of caspases, is a cytosolic enzyme that is synthesized as a pro-caspase consisting of two propeptide (aa 2-23 and 199-206) that are cleaved off by granzyme B or caspase-3 to produce the active caspase-7 that consists of a p20 (aa 24-198) and a p11 (aa 207-303) subunits. In the inactive form these two subunits are connected by a linker sequence that hinders ordering of the active site. The removal of this linker region by cleavage of the peptide bond between Asp198 and Ser199 (in p20) and Asp206 and Ala207 (in p11) activates this enzyme. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Lamkanfi, M., and Kanneganti, TD. (2010). Int. J. Biochem. Cell Biol. 42(1); 21-24).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 20 amino acids from the N-terminal region of human Caspase-7.
Application
Quality Control Testing
Evaluated by Western blotting in lysate from Jurkat cells treated with etoposide.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Caspase-7 in lysates from Jurkat cells overnight serum starved and treated with etoposide (100 μM; 2 h), but not in untreated cells.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Caspase-7 in A549 cell lysate.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Caspase-7 in human small intestine tissue sections.
Flow Cytometry Analysis: 0.1 μg from a representative lot detected Caspase-7 in one million Jurkat cells treated with etoposide.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biochem/physiol Actions
Clone 1E21 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Caspase-7. It targets an epitope within 20 amino acids from the N-terminal region.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1E21, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 34.28 kDa,observed mol wt ~35 kDa |
| purified by | using Protein A |
| species reactivity | human |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | flow cytometry: suitable,immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | N-terminal |
| Protein ID accession no. | NP_001218 |
| UniProt accession no. | P55210 |
| greener alternative category | , Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... CASP7(840) |
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