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General description
Ras-related protein Rab-11A (UniProt: P62491; also known as EC:3.6.5.2, Rab-11, YL8) is encoded by the RAB11A (also known as RAB11) gene (Gene ID: 8766) in human. The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. They cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors that are directly responsible for vesicle formation, movement, tethering and fusion. Rab11 is a lipid-anchored, evolutionarily conserved and ubiquitously expressed membrane protein that is associated with recycling endosomes and has been implicated in regulating vesicular trafficking through recycling of endosomal compartment and early endosomes to the trans- Golgi network and plasma membrane through recycling pathways. The vertebrate Rab11 consists of three members, namely Rab11a, Rab11b, and Rab25, and they have been characterized as regulators of recycling endosomes in both polarized and non-polarized cells. Rab11a acts as a major regulator of membrane delivery during cytokinesis. RAB11A is also reported to bind WD repeat domain phosphoinositide-interacting protein 2 (WIPI2) via a conserved RAB11-binding domain and regulates autophagy. Two isoforms of Rab11a have been described that are produced by alternative splicing. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Puri, C., et al. (2018). Dev. Cell. 45(1); 114-131; Bhuin, T., and Roy, JK. (2015). Int. J. Mol. Cell Med. 4(1); 1-8).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal half of human Rab11.
Application
Quality Control Testing
Evaluated by Western Blotting in SH-SY5Y cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Rab11 in SH-SY5Y cell lysate.
Tested Applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Rab11 in lysates from C2C12 and PC12 cells and Human brain tissue.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Rab11 in Human stomach tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Rab11 in SH-SY5Y cells.
Affinity Binding Assay: A representative lot of this antibody bound Rab11 peptide with a KD of 1.3 x 10-6 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 1C16 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detect Rab11. It targets an epitope within 20 amino acids from the C-terminal half.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | recombinant monoclonal |
| description | clone 1C16 ,recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 24 kDa,observed mol wt ~24 kDa |
| purified by | using Protein A |
| species reactivity | human, mouse, rat |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | C-terminal half |
| Protein ID accession no. | NP_004654 |
| UniProt accession no. | P62491 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Gene Information | human ... RAB11A(8766) |
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