- 商品介绍
- 规格参数
- 包装参数
General description
Azure II Eosinate is the eosinate of the dye Azure II which is a mixture of the dyes methylene blue and azure B in equal proportions. This compound is a key ingredient in Giemsa stain, which is widely utilized for staining blood elements and facilitating the visualization of cellular components under a microscope.
Application
Azure II Eosinate is used for human-medical cell staining and serves the purpose of the investigation of sample material of human origin (by fixing, embedding where necessary, staining, counterstaining, mounting) in hematological and clinico-cytological specimen materials, for example smears of whole blood and bone marrow.
Biochem/physiol Actions
The staining of the nuclei is due of the molecular interaction of the Eosin Y dye and a complex of Azure B with DNA. Both dyes assemble to an Eosin Y- Azure B-DNA complex and the intensity of the resulting stain depends on the content of Azure B and the ratio of Azure B : Eosin Y. Furthermore, the resulting stain can vary depending on the influence of fixation, staining times, pH-value of the solutions or buffer substances.
Features and Benefits
Chief ingredient of Giemsa Stain.
| form | powder |
| Quality Segment | 100 |
| technique(s) | microbe id | staining: suitable |
| color | dark green |
| solubility | soluble, hazy to turbid |
| Amax | ≥0.50 at 644-655 nm in methanol at 0.005 g/L |
| application(s) | diagnostic assay manufacturing hematology histology |
| storage temp. | room temp |
| SMILES string | [Na+].[Na+].[Cl-].[Cl-].CNc1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C.CN(C)c4ccc5N=C6C=C\C(C=C6Sc5c4)=[N+](/C)C.[O-]C(=O)c7ccccc7C8=C9C=C(Br)C(=O)C(Br)=C9Oc%10c(Br)c([O-])c(Br)cc8%10 |
| InChI | 1S/C20H8Br4O5.C16H18N3S.C15H15N3S.2ClH.2Na/c21-11-5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(22)17(26)15(24)19(10)29-18(9)14(23)16(11)25;1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;1-16-10-4-6-12-14(8-10)19-15-9-11(18(2)3)5-7-13(15)17-12;;;;/h1-6,25H,(H,27,28);5-10H,1-4H3;4-9H,1-3H3;2*1H;;/q;+1;;;;2*+1/p-3 |
| InChI key | CSDDIABEPMTRIP-UHFFFAOYSA-K |
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¥ 2814.26
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¥ 1779.22
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¥ 6330.00
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¥ 2268.97
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¥ 25801.21
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