- 商品介绍
- 规格参数
- 包装参数
General description
Transcription factor MafB (UniProt: Q9Y5Q3; also known as V-maf musculoaponeurotic fibrosarcoma oncogene homolog B, Maf-B) is encoded by the MAFB (also known as KRML) gene (Gene ID: 9935) in human. Maf-B is a member of the large Maf family of transcription factors, characterized by a basic leucine zipper (bZIP) domain that facilitates DNA binding and dimerization. This structure enables Maf-B to bind to MARE (Maf recognition elements) sequences within the DNA, allowing it to regulate gene expression. Maf-B can form both homodimers and heterodimers with other bZIP transcription factors, enhancing its regulatory versatility. As a transcription factor, Maf-B plays a critical role in the regulation of gene expression involved in cell differentiation and development. It is particularly important in the differentiation of hematopoietic cells, including monocytes and macrophages, and is also involved in the development of kidney podocytes and islet β cells in the pancreas. Maf-B influences cell cycle regulation, apoptosis, and cellular differentiation processes by activating or repressing target genes. MafB expression is tissue-specific, predominantly found in monocytes, macrophages, and kidney. Its expression is also noted during certain developmental stages, particularly in embryogenesis where it is critical for the proper development of various organs and tissues. In adult tissues, Maf-B expression continues to play a crucial role in maintaining the function and development of specific cell types. In the hematopoietic system, alterations in Maf-B expression are associated with leukemias and other hematological disorders. In the kidney, Maf-B dysfunction can contribute to renal diseases, including glomerulosclerosis. Maf-B′s role in pancreatic β cells also links it to diabetes. Additionally, Maf-B has been implicated in the development of obesity through its regulatory effects on adipogenesis. This ZooMAb® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 17 amino acids from the N-terminal half of human Maf-B.
Application
Quality Control Testing
Evaluated by Western Blotting in SK-N-MC cell lysate.
Western Blotting Analysis (WB): A 1:10,000 dilution of this antibody detected Maf-B in SK-N-MC cell lysate.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Maf-B in human kidney and human testis tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound Maf-B peptide with at least ten thousand-fold (10,000X) higher than with non-specific control peptide.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Maf-B in SK-N-MC cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 2N17 is a ZooMAb® recombinant rabbit monoclonal antibody that detects Maf-B. It targets an epitope within 17 amino acids from the N-terminal half.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 2N17, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 35.79 kDa,observed mol wt ~43 kDa |
| purified by | using Protein A |
| species reactivity | human |
| species reactivity (predicted by homology) | canine, monkey, bovine, rat, porcine, mouse, rabbit |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgGκ |
| epitope sequence | N-terminal |
| Protein ID accession no. | NP_005452.2 |
| UniProt accession no. | Q9Y5Q3 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Gene Information | human ... MAFB(9935) |
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¥ 4164.19
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