Monoclonal Anti-mobC antibody produced in mouse，100UG，SAB1300503-100UG，Sigma 供应商、价格、厂家、报价、规格参数

产品介绍 Product Description

General description

The mobilization region of the 7.4 kb beta-lactamase plasmid pSJ7.4 from Neisseria gonorrhoeae was characterized. The 3.2 kb HindIII-BamHI fragment of pSJ7.4 was mobilized between Escherichia coli strains by conjugative plasmid RK2. Selected restriction enzyme-generated deletions of this fragment were subcloned in pACYC177 to obtain constructs that were suitable for analysis of the mobilization region. Mating experiments showed that a highly conserved 1.9 kb DNA region within coordinates 4096-5997 is required for mobilization of pSJ7.4. This region contains two genes encoding the mobilization protein MobA, another protein, MobC, putatively involved in mobilization from some hosts, and an intergenic oriT. The 168 bp intergenic sequence also contains the promoters for mobA and mobC in an arrangement that suggests divergent transcription and autoregulation from oriT. The 56 kDa MobA was expressed in E. coli as a (6x)His-Tag fusion protein. Purified MobA specifically induced plasmid relaxation by nicking at the oriT. MobA is exceptional because the N-terminal region alone can mobilize pSJ7.4, albeit at a lower frequency than the full-length protein, even in the absence of MobC. The carboxyl terminal region of MobA did not share homology with other mobilization proteins, but may be involved in promoting efficient transfer of pSJ7.4.

Immunogen

MOBC (AAO45531, )
This antibody was raised using purified His-tagged recombinant full length Neisseria gonorrhoeae plasmid pSJ7.4 mobilization protein C (mobC)and mobilization protein A (mobA).

Physical form

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

技术参数 Specifications

biological source	mouse
Quality Level	100
conjugate	unconjugated
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	19CT.2.8.14, monoclonal
form	buffered aqueous solution
species reactivity	Neisseria gonorrhoeae
technique(s)	indirect ELISA: 1:1000,western blot: 1:100-1:500
isotype	IgG1
NCBI accession no.	AAO45531
UniProt accession no.	Q84AT5
shipped in	dry ice
storage temp.	−20°C
target post-translational modification	unmodified

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