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General description
Dual specificity mitogen-activated protein kinase kinase 3/6 (UniProt: P46734/ P52564; also known as EC:2.7.12.2, MAP kinase kinase 3/6, MAPKK 3, MAPK/ERK kinase 3/6, MEK 3/6, Stress-activated protein kinase kinase 2, SAPK kinase 2/3, SAPKK-2/3, SAPKK2/6) is encoded by the MAP2K3/6(also known as MEK3/6, MKK3/6, PRKMK3/6, SKK2/3) gene (Gene ID: 5606/5608) in human. MKK3/MKK6 are dual specificity kinases that act as essential components of the MAP kinase signal transduction pathways. They catalyze the phosphorylation of a threonine and a tyrosine residue in MAP kinases, such as MAPK14/p38-MAPK. Both MKK3 and MKK6 are known to phosphorylate p38 MAP kinase but do not phosphorylate the related JNK or ERK MAP kinases. Their protein kinase domain is localized in amino acids 64-325 (MKK3) and 53-314 (MKK6). Clone 2E21 is generated using a sequence within the protein kinase domain that is common to both MKK3 and MKK6. MKK3 phosphorylation on serine 218 and threonine 222 by MAP kinase kinase kinases positively its kinase activity. MKK6 is phosphorylated at serine 207 and threonine 211 in response to a variety of cellular stresses, including UV radiation, osmotic shock, hypoxia, and inflammatory cytokines. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Raingeaud, J., et al. (1996). Mol. Cell Biol. 16(3); 1247-1255; Moriguchi, T., et al. (1996). J. Biol. Chem. 271(23); 13675-13679).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of human MKK3.
Application
Quality Control Testing
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected MKK3/MKK6 in HEK293 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected MKK3/MKK6 in Caco2, C2C12, and C6 cell lysates.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MKK3/MKK6 in Caco-2 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected MKK3/MKK6 in Human small intestine tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 2E21 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects MKK3. It targets an epitope within 15 amino acids from the C-terminal half. It also displays reactivity with MKK6.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 2E21, recombinant monoclonal |
| description | 2E21 Clone |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 37.49 kDa,observed mol wt ~38 kDa |
| purified by | using Protein A |
| species reactivity | mouse, human, rat |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| technique(s) | immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | C-terminal half |
| Protein ID accession no. | NP_659731/NP_002749 |
| UniProt accession no. | P46734/P52564 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
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