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General description
Fibrinogen beta chain (UniProt: P02675) is encoded by the FGB gene (Gene ID: 2244) in human. Fibrinogen beta chain is synthesized with a signal peptide (aa 1-30), which is subsequently cleaved off to product the mature protein that is further cleaved by thrombin to produce fibrinopeptide B (aa 31-44) and fibrinogen beta chain (45-491). Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers. Fibrin monomers spontaneously aggregate into an insoluble gel, which is then covalently stabilized by Factor XIIIa. Fibrin is shown to retain about 98% of the original covalent structure of fibrinogen. Fibrin clot can be broken down by plasmin by cleave between residues 152-153 and 163-164. A mouse monoclonal version of this clone binds to fibrin even in the presence of human fibrinogen at the concentration found in plasma. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonal. (Ref.: Litvinov, RI., and Weisel, JW (2016). Semin. Thromb. Hemost. 42(4); 333-343; Hui, KY., et al. (1983). Science. 222 (4628); 1129-1132).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 7 amino acids from the N-terminal region of human Fibrinogen β chain.
Application
Quality Control Testing
Evaluated by Western Blotting in Human plasma.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Fibrin in Human plasma.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Fibrin in lysates from HepG2 cells and from Mouse liver tissue..
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Fibrin in Human placenta tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Fibrin in Human kidney and Human placenta tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 59D8 is a ZooMAb® mouse recombinant monoclonal antibody that specifically detects Fibrin. It targets an epitope within 7 amino acids from the N-terminal region.
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | recombinant monoclonal |
| description | clone 59D8 ,recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 55.93 kDa,observed mol wt ~56 kDa |
| purified by | using protein G |
| species reactivity | mouse, human |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | immunofluorescence: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG1κ |
| epitope sequence | N-terminal |
| Protein ID accession no. | NP_005132.2 |
| UniProt accession no. | P02675 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Gene Information | human ... FGB(2244) |
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