Anti-Human IgG (γ-chain specific), F(ab′)₂ fragment−Alkaline Phosphatase antibody produced in goat,1ML,A5403-1ML,Sigma

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订货号 0BN5031
品牌型号 Sigma A5403-1ML
货期 询货期
最小订货量 1件
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产品介绍 Product Description

General description

Human IgG antibodies are primarily responsible for the facilitation of humoral immune responses such as placental transport, complement fixation and phagocytosis among many others. The antigen binding is mediated by the variable Fab region of IgG antibodies, whereas the effector activities are stimulated by the Fc domain , . Anti-human IgG (γ-chain specific), F(ab′)2 fragment-alkaline phosphatase antibody can be used in ELISA for measuring anticardiolipin antibodies. Goat anti-human IgG (γ-chain specific), F(ab′)2 fragment-alkaline phosphatase antibody reacts specifically with human IgG.


Immunogen

Purified human IgG.


Application

Serum IgGs against HHV-6 or HHV-7 were detected by Elisa using alkaline phosphatase conjugated goat anti-human IgG F′ab specific at a concentration of 1:1000 diluted in PBS/0.05% skim milk. The antibody was incubated on plates for 2 hours at 37 degrees.
Transfected SN56 neuronal cell lysates were ran on a SDS page gel for western blot analysis using alkaline phosphatase conjugated goat anti-human IgG Fab specific as the secondary antibody.


Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide


Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


技术参数 Specifications
biological sourcegoat
Quality Level200
conjugatealkaline phosphatase conjugate
antibody formaffinity isolated antibody
antibody product typesecondary antibodies
clonepolyclonal
formbuffered aqueous solution
technique(s)direct ELISA: 1:1,000
shipped inwet ice
storage temp.2-8°C
target post-translational modificationunmodified
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